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rdf:type |
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lifeskim:mentions |
umls-concept:C0005595,
umls-concept:C0016627,
umls-concept:C0028326,
umls-concept:C0029347,
umls-concept:C0033684,
umls-concept:C0040711,
umls-concept:C0086418,
umls-concept:C0178539,
umls-concept:C0205250,
umls-concept:C0205251,
umls-concept:C0443199,
umls-concept:C0450254,
umls-concept:C0949765,
umls-concept:C1280500,
umls-concept:C1615608,
umls-concept:C1706044
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pubmed:issue |
9
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pubmed:dateCreated |
2011-3-1
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pubmed:abstractText |
The nonstructural protein NS1 of influenza A virus blocks the development of host antiviral responses by inhibiting polyadenylation of cellular pre-mRNA. NS1 also promotes the synthesis of viral proteins by stimulating mRNA translation. Here, we show that recombinant NS1 proteins of human pandemic H1N1/2009, avian highly pathogenic H5N1, and low pathogenic H5N2 influenza strains differentially affected these two cellular processes: NS1 of the two avian strains, in contrast to NS1 of H1N1/2009, stimulated translation of reporter mRNA in cell-free translation system; NS1 of H5N1 was an effective inhibitor of cellular pre-mRNA polyadenylation in A549 cells, unlike NS1 of H5N2 and H1N1/2009. We identified key amino acids in NS1 that contribute to its activity in these two basic cellular processes. Thus, we identified strain-specific differences between influenza virus NS1 proteins in pre-mRNA polyadenylation and mRNA translation.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
1083-351X
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:day |
4
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pubmed:volume |
286
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
7239-47
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pubmed:meshHeading |
pubmed-meshheading:21163951-Amino Acid Sequence,
pubmed-meshheading:21163951-Animals,
pubmed-meshheading:21163951-Humans,
pubmed-meshheading:21163951-Influenza A Virus, H1N1 Subtype,
pubmed-meshheading:21163951-Influenza A Virus, H5N1 Subtype,
pubmed-meshheading:21163951-Influenza A Virus, H5N2 Subtype,
pubmed-meshheading:21163951-Influenza A virus,
pubmed-meshheading:21163951-Models, Chemical,
pubmed-meshheading:21163951-Molecular Sequence Data,
pubmed-meshheading:21163951-Orthomyxoviridae Infections,
pubmed-meshheading:21163951-Pandemics,
pubmed-meshheading:21163951-Polyadenylation,
pubmed-meshheading:21163951-Protein Biosynthesis,
pubmed-meshheading:21163951-Protein Stability,
pubmed-meshheading:21163951-Protein Structure, Tertiary,
pubmed-meshheading:21163951-RNA Precursors,
pubmed-meshheading:21163951-Species Specificity,
pubmed-meshheading:21163951-Viral Nonstructural Proteins,
pubmed-meshheading:21163951-Virulence
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pubmed:year |
2011
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pubmed:articleTitle |
Differential effects of NS1 proteins of human pandemic H1N1/2009, avian highly pathogenic H5N1, and low pathogenic H5N2 influenza A viruses on cellular pre-mRNA polyadenylation and mRNA translation.
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pubmed:affiliation |
Institute of Immunology, Centre de Recherche Public-Santé/Laboratoire National de Santé, L-1011, Luxembourg.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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