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PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2010-12-16
pubmed:abstractText
We examined the prevalence of antibodies to zoonotic protozoan parasites ( Trypanosoma cruzi, Toxoplasma gondii, and Encephalitozoon cuniculi) and protozoans of veterinary importance ( Neospora caninum, Sarcocystis neurona, and Besnoitia darlingi) in a population of North American opossums ( Didelphis virginiana) from Louisiana. Samples from 30 opossums were collected as part of a survey for T. cruzi in Louisiana. Frozen sera from these 30 opossums were examined using an indirect immunofluorescent antibody test (IFAT) against in vitro-produced antigenic stages of these protozoans. Additionally, 24 of the 30 samples were examined using hemoculture, and all 30 were examined in the modified direct agglutination test (MAT) for antibodies to To. gondii. The prevalences of reactive IFAT samples were as follows: 60% for T. cruzi, 27% for To. gondii, 23% for E. cuniculi, 17% for S. neurona, 47% for B. darlingi, and 0% for N. caninum. Hemoculture revealed that 16 (67%) of 24 samples were positive for T. cruzi, compared to 18 of 30 (60%) by IFAT. The sensitivity and specificity for the IFAT compared to hemoculture was 100% for each. The modified direct agglutination test revealed that 9 (30%) of the 30 samples from opossums had antibodies to To. gondii , compared to 8 (27%) using the IFAT. The sensitivity and specificity of the IFAT compared to the MAT was 100% and 72%, respectively.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
1937-2345
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
96
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1119-22
pubmed:meshHeading
pubmed-meshheading:21158620-Agglutination Tests, pubmed-meshheading:21158620-Animals, pubmed-meshheading:21158620-Antibodies, Fungal, pubmed-meshheading:21158620-Antibodies, Protozoan, pubmed-meshheading:21158620-Chagas Disease, pubmed-meshheading:21158620-Coccidiosis, pubmed-meshheading:21158620-Didelphis, pubmed-meshheading:21158620-Encephalitozoon cuniculi, pubmed-meshheading:21158620-Encephalitozoonosis, pubmed-meshheading:21158620-Fluorescent Antibody Technique, Indirect, pubmed-meshheading:21158620-Louisiana, pubmed-meshheading:21158620-Neospora, pubmed-meshheading:21158620-Protozoan Infections, Animal, pubmed-meshheading:21158620-Sarcocystidae, pubmed-meshheading:21158620-Sarcocystis, pubmed-meshheading:21158620-Sarcocystosis, pubmed-meshheading:21158620-Sensitivity and Specificity, pubmed-meshheading:21158620-Seroepidemiologic Studies, pubmed-meshheading:21158620-Toxoplasma, pubmed-meshheading:21158620-Toxoplasmosis, Animal, pubmed-meshheading:21158620-Trypanosoma cruzi, pubmed-meshheading:21158620-Zoonoses
pubmed:year
2010
pubmed:articleTitle
Prevalence of antibodies to Trypanosoma cruzi, Toxoplasma gondii, Encephalitozoon cuniculi, Sarcocystis neurona, Besnoitia darlingi, and Neospora caninum in North American opossums, Didelphis virginiana, from southern Louisiana.
pubmed:affiliation
Department of Biomedical Sciences and Pathobiology, Virginia-Maryland Regional College of Veterinary Medicine, Virginia Tech, Blacksburg, Virginia 24061-0342, USA.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't