Linked Life Data

21154552

Source:http://linkedlifedata.com/resource/pubmed/id/21154552

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2010-12-14
pubmed:abstractText
Correct localization and topology are crucial for a protein's cellular function. To determine topologies of membrane proteins, a new technique, called fluorescence protease protection (FPP) assay, has recently been established. The sole requirements for FPP are the expression of fluorescent-protein fusion proteins and the selective permeabilization of the plasma membrane, permitting a wide range of cell types and organelles to be investigated. Proteins topologies in organelles like endoplasmic reticulum, Golgi apparatus, mitochondria, peroxisomes, and autophagosomes have already been determined by FPP. Here, two different step-by-step protocols of the FPP assay are provided. First, we describe the FPP assay using fluorescence microscopy for single adherent cells, and second, we outline the FPP assay for high-throughput screening applications.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
1934-2616
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
Chapter 5
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
Unit 5.7
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
Determining membrane protein topologies in single cells and high-throughput screening applications.
pubmed:affiliation
National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland, USA.
pubmed:publicationType
Journal Article