Linked Life Data

21150134

Source:http://linkedlifedata.com/resource/pubmed/id/21150134

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2011-4-28
pubmed:abstractText
Embryonated chicken eggs (ECEs) are routinely used to isolate equine influenza virus. Propagation of the virus in ECEs results in selection of variants. In the present study, we determined nucleotide sequences of entire coding regions of parent A/equine/Tottori/1/07 (H3N8) and its derivatives that have different passage histories in ECE. After 12 passages, nucleotide sequence analysis predicted 3 amino acid substitutions in hemagglutinin (HA; 2 in HA1 and 1 in HA2). The two amino acid substitutions in HA1 were located in the vicinity of the cell receptor-binding site. Three other amino acid substitutions were predicted in internal proteins, 1 in the M1, 1 in the NP and 1 in the PA. This is the first report showing mutations in the internal protein genes of equine influenza virus associated with adaptation to ECE.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0916-7250
pubmed:author
pubmed:issnType
Print
pubmed:volume
73
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
545-8
pubmed:meshHeading
pubmed:year
2011
pubmed:articleTitle
Molecular changes associated with adaptation of equine influenza H3N8 virus in embryonated chicken eggs.
pubmed:affiliation
Laboratory of Veterinary Hygiene, Department of Veterinary Medicine, Faculty of Agriculture, Tottori University, Tottori, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't