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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
2011-1-7
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pubmed:abstractText |
The redox-midpoint potential of the FAD chromophore in the BLUF domain of anti-transcriptional regulator AppA from Rhodobacter sphaeroides equals ?-260mV relative to the calomel electrode. Altering the structure of its chromophore-binding pocket through site-directed mutagenesis brings this midpoint potential closer to that of free flavin in aqueous solution. The redox-midpoint potential of this BLUF domain is intermediate between those of LOV domains and Cryptochromes, which may rationalize the primary photochemistry observed in these three flavin-containing photoreceptor families. These results also imply that LOV domains, among the flavin-containing photosensory receptors, are least sensitive to intracellular chemical reduction in the dark.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
1873-3468
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pubmed:author |
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pubmed:copyrightInfo |
Copyright © 2010 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
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pubmed:issnType |
Electronic
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pubmed:day |
3
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pubmed:volume |
585
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
167-72
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pubmed:meshHeading |
pubmed-meshheading:21110976-Algorithms,
pubmed-meshheading:21110976-Amino Acid Sequence,
pubmed-meshheading:21110976-Bacterial Proteins,
pubmed-meshheading:21110976-Binding Sites,
pubmed-meshheading:21110976-Cryptochromes,
pubmed-meshheading:21110976-Flavin-Adenine Dinucleotide,
pubmed-meshheading:21110976-Flavins,
pubmed-meshheading:21110976-Flavoproteins,
pubmed-meshheading:21110976-Kinetics,
pubmed-meshheading:21110976-Light,
pubmed-meshheading:21110976-Mutagenesis, Site-Directed,
pubmed-meshheading:21110976-Oxidation-Reduction,
pubmed-meshheading:21110976-Oxygen,
pubmed-meshheading:21110976-Photoreceptors, Microbial,
pubmed-meshheading:21110976-Rhodobacter sphaeroides,
pubmed-meshheading:21110976-Spectrophotometry
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pubmed:year |
2011
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pubmed:articleTitle |
On the midpoint potential of the FAD chromophore in a BLUF-domain containing photoreceptor protein.
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pubmed:affiliation |
Swammerdam Institute for Life Science, University of Amsterdam, Amsterdam, The Netherlands.
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pubmed:publicationType |
Journal Article
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