Linked Life Data

21109589

Source:http://linkedlifedata.com/resource/pubmed/id/21109589

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2011-1-31
pubmed:abstractText
Epithelial proliferation, critical for homeostasis, healing, and colon cancer progression, is in part controlled by epidermal growth factor receptor (EGFR). Proliferation of colonic epithelia can be induced by Citrobacter rodentium infection, and we have demonstrated that activity of tumor suppressor FOXO3 was attenuated after this infection. Thus the aim of this study was to determine the contribution of FOXO3 in EGFR-dependent proliferation of intestinal epithelia and colon cancer cell lines. In this study we show that, during infection with C. rodentium, EGFR was significantly phosphorylated in colonic mucosa and Foxo3 deficiency in this model lead to an increased number of bromodeoxyuridine-positive cells. In vitro, in human colon cancer cells, increased expression and activation of EGFR was associated with proliferation that leads to FOXO3 phosphorylation (inactivation). Following EGFR activation, FOXO3 was phosphorylated (via phosphatidylinositol 3-kinase/Akt) and translocated to the cytosol where it was degraded. Moreover, inhibition of proliferation by overexpressing FOXO3 was not reversed by the EGFR signaling, implicating FOXO3 as one of the regulators downstream of EGFR. FOXO3 binding to the promoter of the cell cycle inhibitor p27kip1 was decreased by EGFR signaling, suggesting its role in EGFR-dependent proliferation. In conclusion, we show that proliferation in colonic epithelia and colon cancer cells, stimulated by EGFR, is mediated via loss of FOXO3 activity and speculate that FOXO3 may serve as a target in the development of new pharmacological treatments of proliferative diseases.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
1522-1547
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
300
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
G264-72
pubmed:meshHeading
pubmed-meshheading:21109589-Animals, pubmed-meshheading:21109589-Cell Cycle, pubmed-meshheading:21109589-Cell Line, Tumor, pubmed-meshheading:21109589-Cell Proliferation, pubmed-meshheading:21109589-Colon, pubmed-meshheading:21109589-Colonic Neoplasms, pubmed-meshheading:21109589-Cyclin-Dependent Kinase Inhibitor p27, pubmed-meshheading:21109589-Cytosol, pubmed-meshheading:21109589-Down-Regulation, pubmed-meshheading:21109589-Forkhead Transcription Factors, pubmed-meshheading:21109589-Humans, pubmed-meshheading:21109589-Intestinal Mucosa, pubmed-meshheading:21109589-Mice, pubmed-meshheading:21109589-Mice, Knockout, pubmed-meshheading:21109589-Phosphatidylinositol 3-Kinases, pubmed-meshheading:21109589-Phosphorylation, pubmed-meshheading:21109589-Proto-Oncogene Proteins c-akt, pubmed-meshheading:21109589-Receptor, Epidermal Growth Factor, pubmed-meshheading:21109589-Signal Transduction
pubmed:year
2011
pubmed:articleTitle
Tumor suppressor FOXO3 mediates signals from the EGF receptor to regulate proliferation of colonic cells.
pubmed:affiliation
NorthShore Univ. Research Institute, Dept. of Medicine, Div. of Gastroenterology, 1001 University Pl.; Rm. 314, Evanston, IL 60201, USA.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't, Research Support, N.I.H., Extramural