Linked Life Data

2110948

Source:http://linkedlifedata.com/resource/pubmed/id/2110948

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1990-6-20
pubmed:abstractText
Glutaraldehyde (GA) is commonly used to achieve the stable fixation of whole cells onto microtitre plates for use in solid-phase ELISA. Concentrations of GA greater than 0.10% (v/v) were found to result in increased levels of non-specific binding of antibodies in ELISA. However, the use of 0.05-0.025% (v/v) GA permitted the stable fixation of cells whilst minimizing the non-specific effects seen with higher GA concentrations. The binding of cells onto microtitre plates was also found to be highly variable depending on both the type of plates and the nature of the cells being used.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0022-1759
pubmed:author
pubmed:issnType
Print
pubmed:day
8
pubmed:volume
129
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
71-6
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1990
pubmed:articleTitle
Optimal fixation of cells for use in solid-phase ELISA.
pubmed:affiliation
BioResearch Ireland, Dublin.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't