Linked Life Data

2110832

Source:http://linkedlifedata.com/resource/pubmed/id/2110832

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1990-6-21
pubmed:abstractText
The luminescent lanthanides are potentially useful probes of cation-induced events involving phospholipid membranes. In this work, the spectroscopic properties of Tb3+, Ce3+ and Eu3+ are shown to be complementary in defining three forms of complex with phosphatidic acid vesicles. Ce3+, in particular, is useful for studying dilute cation-lipid complexes because it has strong excitation bands in the near ultraviolet. In addition to providing a means for detecting chemically distinct forms of lanthanide-lipid complexes, the luminescence can be used to monitor cation-induced lateral segregation. Ce3+ to Tb3+ energy transfer was observed at lanthanide levels as low as 1:1000 Ln3+/phosphatidic acid, indicating clustering or phase separation. Initial clustering occurs on a subsecond timescale, followed by a much slower aggregation continuing for several minutes to hours. Addition of a chelator results in slow release of the lanthanides. In the case of the dioleoylphosphatidic acid complexes, release is bimodal and indicative of cation entrapment; dimyristoylphosphatidic acid complexes exhibit this behavior only at high temperatures. These observations are consistent with the relative tendencies of these two lipids to form the HII phase. This work sets the foundation for experiments designed to determine the size of nucleation sites for cation-induced events such as intramembrane inverted micelle formation and membrane fusion.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0006-3002
pubmed:author
pubmed:issnType
Print
pubmed:day
9
pubmed:volume
1024
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
159-66
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed:year
1990
pubmed:articleTitle
Lanthanide(III)-phosphatidic acid complexes: binding site heterogeneity and phase separation.
pubmed:affiliation
Department of Chemistry, Seton Hall University, South Orange, NJ 07079.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't