Linked Life Data

21099449

Source:http://linkedlifedata.com/resource/pubmed/id/21099449

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2011-2-8
pubmed:abstractText
Candida albicans is the leading fungal pathogen causing invasive disease in immunocompromised patients including the neonate. A reliable animal model for disseminated candidiasis in the neonate is needed to study the unique aspects of this host-pathogen interaction. To establish such a model, 2-d-old BALB/c mouse pups were given i.p. injections with varied inocula of C. albicans or saline control. Pups were examined every 3-8 h for death. Surviving pups were killed at 72 h. Kidney, lung, spleen, liver, and brain were homogenized and plated for colony counts and/or fixed for histological staining. The i.p. injection of C. albicans led to mortality in a dose-dependent fashion. Disseminated infection was confirmed by colony counts of homogenized kidney, lung, and brain, as well as by histological examination. Infection with a C. albicans mutant lacking the cell surface adhesin, Als3p, led to significant reduction in mortality relative to WT (p = 0.03). This model will be useful to study the unique aspects of antifungal defense in a neonatal host and will provide a means to test novel therapeutic strategies.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
1530-0447
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
69
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
189-93
pubmed:meshHeading
pubmed:year
2011
pubmed:articleTitle
A murine model for disseminated candidiasis in neonates.
pubmed:affiliation
Department of Pediatrics, Women & Infants Hospital of Rhode Island, Warren Alpert Medical School of Brown University, Providence, Rhode Island 02905, USA.
pubmed:publicationType
Journal Article, Research Support, N.I.H., Extramural