Source:http://linkedlifedata.com/resource/pubmed/id/21093010
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
3
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pubmed:dateCreated |
2011-1-3
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pubmed:abstractText |
Exposure to human pathogenic viruses in recreational waters has been shown to cause disease outbreaks. In the context of Article 14 of the revised European Bathing Waters Directive 2006/7/EC (rBWD, CEU, 2006) a Europe-wide surveillance study was carried out to determine the frequency of occurrence of two human enteric viruses in recreational waters. Adenoviruses were selected based on their near-universal shedding and environmental survival, and noroviruses (NoV) selected as being the most prevalent gastroenteritis agent worldwide. Concentration of marine and freshwater samples was done by adsorption/elution followed by molecular detection by (RT)-PCR. Out of 1410 samples, 553 (39.2%) were positive for one or more of the target viruses. Adenoviruses, detected in 36.4% of samples, were more prevalent than noroviruses (9.4%), with 3.5% GI and 6.2% GII, some samples being positive for both GI and GII. Of 513 human adenovirus-positive samples, 63 (12.3%) were also norovirus-positive, whereas 69 (7.7%) norovirus-positive samples were adenovirus-negative. More freshwater samples than marine water samples were virus-positive. Out of a small selection of samples tested for adenovirus infectivity, approximately one-quarter were positive. Sixty percent of 132 nested-PCR adenovirus-positive samples analysed by quantitative PCR gave a mean value of over 3000 genome copies per L of water. The simultaneous detection of infectious adenovirus and of adenovirus and NoV by (RT)PCR suggests that the presence of infectious viruses in recreational waters may constitute a public health risk upon exposure. These studies support the case for considering adenoviruses as an indicator of bathing water quality.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
1879-2448
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pubmed:author |
pubmed-author:CarducciAnnalauraA,
pubmed-author:CookNigelN,
pubmed-author:D'AgostinoMartinM,
pubmed-author:DiviziaMaurizioM,
pubmed-author:FleischerJensJ,
pubmed-author:GantzerChristopheC,
pubmed-author:GawlerAndrewA,
pubmed-author:GironesRosinaR,
pubmed-author:HöllerChristianeC,
pubmed-author:KayDavidD,
pubmed-author:KozyraIwonaI,
pubmed-author:López-PilaJuanJ,
pubmed-author:MuscilloMicheleM,
pubmed-author:NascimentoMaria São JoséMS,
pubmed-author:PapageorgiouGeorgeG,
pubmed-author:RutjesSaskiaS,
pubmed-author:SellwoodJaneJ,
pubmed-author:SzewzykRegineR,
pubmed-author:WyerMarkM,
pubmed-author:Wyn-JonesA PeterAP,
pubmed-author:de Roda HusmanAna MariaAM
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pubmed:copyrightInfo |
Copyright © 2010 Elsevier Ltd. All rights reserved.
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pubmed:issnType |
Electronic
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pubmed:volume |
45
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1025-38
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pubmed:meshHeading |
pubmed-meshheading:21093010-Adenoviridae,
pubmed-meshheading:21093010-Environmental Monitoring,
pubmed-meshheading:21093010-Europe,
pubmed-meshheading:21093010-Fresh Water,
pubmed-meshheading:21093010-Norovirus,
pubmed-meshheading:21093010-Recreation,
pubmed-meshheading:21093010-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:21093010-Seawater,
pubmed-meshheading:21093010-Water Microbiology
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pubmed:year |
2011
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pubmed:articleTitle |
Surveillance of adenoviruses and noroviruses in European recreational waters.
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pubmed:affiliation |
IGES, University of Aberystwyth, Ceredigion, SY23 3DB, UK. pyw@aber.ac.uk
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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