Linked Life Data

21084383

Source:http://linkedlifedata.com/resource/pubmed/id/21084383

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2011-1-3
pubmed:abstractText
The pituitary gland contains six distinct hormone-secreting cell types that are essential for basic physiological processes including fertility and responding to stress. Formation of hormone-secreting cells during development relies on Notch signaling to prevent progenitors from prematurely differentiating. The nature of the signal curtailing Notch signaling in the pituitary is unknown, but a good candidate is the endocytic adaptor protein NUMB. NUMB targets Notch for proteolytic degradation, but it also has a broad range of actions, including stabilizing adherens junctions through interactions with cadherins and influencing cell proliferation by stabilizing expression of the tumor suppressor protein p53. Here, we show that NUMB and its closely related homolog, NUMBLIKE, are expressed in undifferentiated cells during development and later in gonadotropes in the anterior lobe and melanotropes of the intermediate lobe. All four isoforms of NUMB, are detectable in the pituitary, with the shorter forms becoming more prominent after adolescence. Conditionally deleting Numb and Numblike in the intermediate lobe melanotropes with Pomc Cre mice dramatically alters the morphology of cells in the intermediate lobe, coincident with impaired localization of adherens junctions proteins including E-CADHERIN, N-CADHERIN, ?-CATENIN, and ?-CATENIN. Strikingly, the border between posterior and intermediate lobes is also disrupted. These mice also have disorganized progenitor cells, marked by SOX2, but proliferation is unaffected. Unexpectedly, Notch activity appears normal in conditional knockout mice. Thus, Numb is critical for maintaining cell-cell interactions in the pituitary intermediate lobe that are essential for proper cell placement.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
1944-9917
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
25
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
117-27
pubmed:meshHeading
pubmed-meshheading:21084383-Animals, pubmed-meshheading:21084383-Cell Adhesion, pubmed-meshheading:21084383-Cell Movement, pubmed-meshheading:21084383-Cell Proliferation, pubmed-meshheading:21084383-Gene Deletion, pubmed-meshheading:21084383-Gene Expression Regulation, Developmental, pubmed-meshheading:21084383-Gonadotrophs, pubmed-meshheading:21084383-Integrases, pubmed-meshheading:21084383-Melanotrophs, pubmed-meshheading:21084383-Membrane Proteins, pubmed-meshheading:21084383-Mice, pubmed-meshheading:21084383-Mice, Knockout, pubmed-meshheading:21084383-Nerve Tissue Proteins, pubmed-meshheading:21084383-Pituitary Gland, Intermediate, pubmed-meshheading:21084383-Pro-Opiomelanocortin, pubmed-meshheading:21084383-Protein Isoforms, pubmed-meshheading:21084383-Receptors, Notch, pubmed-meshheading:21084383-Stem Cells
pubmed:year
2011
pubmed:articleTitle
Numb deletion in POMC-expressing cells impairs pituitary intermediate lobe cell adhesion, progenitor cell localization, and neuro-intermediate lobe boundary formation.
pubmed:affiliation
University of Illinois at Urbana-Champaign, 407 South Goodwin Avenue, Urbana, Illinois 61801, USA.
pubmed:publicationType
Journal Article, Research Support, N.I.H., Extramural