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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1990-3-1
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pubmed:abstractText |
The effect of differentiation on FMLP-stimulated InsP production and G-protein expression was investigated in U937 monocytes. FMLP (0.01-10 microM) stimulated [3H]InsP production in dimethyl sulphoxide-differentiated, but not in immature, U937 cells. Ionomycin (1 and 10 microM) stimulated [3H]InsP production equally well in both cell types. The FMLP response was blocked by pertussis toxin (100 ng/ml for 4 h) which catalysed [32P]ADP ribosylation of a 40 kDa 'Gi-like' G-protein alpha subunit in these cells. This protein was also identified immunologically using anti-peptide antibodies that detect 'Gi-like' alpha subunits (SG2) or Gi2 alpha specifically (LE2). With LE2 a 5-fold increase in Gi2 alpha levels was seen following differentiation of the cells, suggesting that FMLP receptor expression is accompanied by an increase in the G-protein with which these receptors interact.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0006-3002
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
23
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pubmed:volume |
1051
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
71-7
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pubmed:dateRevised |
2004-11-17
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pubmed:meshHeading |
pubmed-meshheading:2105104-Adenosine Diphosphate Ribose,
pubmed-meshheading:2105104-Cell Differentiation,
pubmed-meshheading:2105104-Cell Line,
pubmed-meshheading:2105104-Chromatography, High Pressure Liquid,
pubmed-meshheading:2105104-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:2105104-GTP-Binding Proteins,
pubmed-meshheading:2105104-Humans,
pubmed-meshheading:2105104-Inositol Phosphates,
pubmed-meshheading:2105104-Ionomycin,
pubmed-meshheading:2105104-Monocytes,
pubmed-meshheading:2105104-N-Formylmethionine Leucyl-Phenylalanine,
pubmed-meshheading:2105104-Pertussis Toxin,
pubmed-meshheading:2105104-Virulence Factors, Bordetella
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pubmed:year |
1990
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pubmed:articleTitle |
Stimulus-response coupling in FMLP-stimulated U937 monocytes: effect of differentiation on Gi2 expression.
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pubmed:affiliation |
Bioscience II, ICI Pharmaceuticals, Alderley Park, Glasgow, U.K.
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pubmed:publicationType |
Journal Article
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