Linked Life Data

20979535

Source:http://linkedlifedata.com/resource/pubmed/id/20979535

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
7-8
pubmed:dateCreated
2011-3-23
pubmed:abstractText
Transfection with either hypoxia-inducible factor-1? (HIF-1?) or heme oxygenase-1 (HO-1) gene can induce neovascularization in ischemic tissues. Although expression of transfected HIF-1? gene occurs rapidly, the expressed HIF-1? protein degrades quickly, limiting its therapeutic efficacy. Meanwhile, expressed HO-1 protein does not rapidly undergo degradation, but gene expression occurs a couple of days after transfection, resulting in apoptosis and a delay in angiogenesis in ischemic tissues at the incipient period of HO-1 gene transfection. We hypothesize that combined delivery of HIF-1? and HO-1 gene will enhance antiapoptosis and neovascularization in ischemic tissue compared with HIF-1? or HO-1 single-gene therapy. To test this hypothesis, ischemic mouse hindlimbs were treated with HIF-1? and/or HO-1 gene therapy. The combined gene therapy proved superior to both single-gene therapies, resulting in rapid expression of HIF-1? gene and long-term maintenance of expressed HO-1 protein. The apoptosis in the ischemic region was significantly less, and angiogenic growth factor secretion and angiogenesis were greater in the combined gene therapy than in either of the single-gene therapies. Our results suggest that a combined gene therapy of HIF-1? and HO-1 enhances the transfection of both genes and improves angiogenesis compared with either single-gene therapy.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
1937-335X
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
17
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
915-26
pubmed:meshHeading
pubmed:year
2011
pubmed:articleTitle
Combined gene therapy with hypoxia-inducible factor-1? and heme oxygenase-1 for therapeutic angiogenesis.
pubmed:affiliation
School of Chemical and Biological Engineering, Seoul National University, Seoul, Republic of Korea.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't