Linked Life Data

20976199

Source:http://linkedlifedata.com/resource/pubmed/id/20976199

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
10
pubmed:dateCreated
2010-10-26
pubmed:abstractText
Hair cell stereocilia structure depends on actin filaments composed of cytoplasmic ?-actin and ?-actin isoforms. Mutations in either gene can lead to progressive hearing loss in humans. Since ?-actin and ?-actin isoforms are 99% identical at the protein level, it is unclear whether each isoform has distinct cellular roles. Here, we compared the functions of ?-actin and ?-actin in stereocilia formation and maintenance by generating mice conditionally knocked out for Actb or Actg1 in hair cells. We found that, although cytoplasmic actin is necessary, neither ?-actin nor ?-actin is required for normal stereocilia development or auditory function in young animals. However, aging mice with ?-actin- or ?-actin-deficient hair cells develop different patterns of progressive hearing loss and distinct pathogenic changes in stereocilia morphology, despite colocalization of the actin isoforms. These results demonstrate overlapping developmental roles but unique post-developmental functions for ?-actin and ?-actin in maintaining hair cell stereocilia.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
1553-7404
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
6
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
e1001158
pubmed:dateRevised
2011-3-11
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
?-actin and ?-actin are each dispensable for auditory hair cell development but required for Stereocilia maintenance.
pubmed:affiliation
Department of Biochemistry, Molecular Biology, and Biophysics, University of Minnesota, Minneapolis, Minnesota, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't, Research Support, N.I.H., Extramural