Linked Life Data

20973117

Source:http://linkedlifedata.com/resource/pubmed/id/20973117

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
16
pubmed:dateCreated
2010-11-8
pubmed:abstractText
Herein the reactivity of the enzyme ?-chymotrypsin in the confinement of polystyrene-block-poly(acrylic acid) (PS-b-PAA) vesicles was investigated. Enzyme and substrate molecules were encapsulated in PS-b-PAA vesicles with internal diameters ranging from 26 nm to 165 nm during the formation of the vesicles. While the loading efficiencies of enzyme and substrate molecules were practically identical for vesicles of identical size, they were found to increase with decreasing vesicle size. The kinetics of the ?-chymotrypsin catalyzed hydrolysis of N-succinyl-Ala-Ala-Phe-7-amido-4-methylcoumarin (AMC) was evaluated following the increase of the absorption of the product 7-amino-4-methylcoumarin by UV/Vis spectroscopy. The values of the catalytic turnover number obtained for reactions inside vesicles with different sizes showed an increase of up to fourteen times compared to the bulk value with decreasing vesicle volume, while the values of the Michaelis-Menten constant decreased, respectively. This increase in reactivity of ?-chymotrypsin is attributed to the effect of vesicle-wall interactions in the finite encapsulated space, where the reagents could diffuse, leading to enhanced collision frequencies.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
1439-7641
pubmed:author
pubmed:issnType
Electronic
pubmed:day
15
pubmed:volume
11
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3534-40
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
?-Chymotrypsin-catalyzed reaction confined in block-copolymer vesicles.
pubmed:affiliation
Department of Materials Science and Technology of Polymers, University of Twente, MESA+Institute for Nanotechnology, PO Box 217, 7500 AE Enschede, The Netherlands.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't