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pubmed-article:20970930pubmed:abstractTextHigh rates of mortality for Pekin ducklings have been recorded in several duck farms in China since 2006. Dead ducklings were characterized by spleen necrosis, suggesting microbial infection as a cause of disease. Laboratory investigations led to the isolation of a virus strain from the spleen tissues of dead ducklings, designated DRV-HC. Subsequent experimental infections with DRV-HC resulted in marked spleen necrosis in the ducklings similar to those observed in the natural outbreaks. Electron microscopy of the cultured DRV-HC revealed viral particles that were non-enveloped and icosahedral with a mean diameter of approximately 72 nm. Agar gel precipitating tests showed that the isolate shared a common group-specific antigen with chicken reovirus S1133. DNA sequencing revealed that this isolate was closely related to Muscovy duck reoviruses. Experimental infection with DRV-HC resulted in death of young chicks with necrotic foci in the liver and spleen. To the best of our knowledge, this is the first report of the isolation of a duck reovirus with high virulence in Pekin ducklings and SPF chickens.lld:pubmed
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pubmed-article:20970930pubmed:authorpubmed-author:HuangYuYlld:pubmed
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pubmed-article:20970930pubmed:authorpubmed-author:ZhangDabingDlld:pubmed
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pubmed-article:20970930pubmed:authorpubmed-author:LiuQinfangQlld:pubmed
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pubmed-article:20970930pubmed:authorpubmed-author:ChenLibenLlld:pubmed
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pubmed-article:20970930pubmed:authorpubmed-author:SuWenliangWlld:pubmed
pubmed-article:20970930pubmed:copyrightInfoCopyright © 2010 Elsevier B.V. All rights reserved.lld:pubmed
pubmed-article:20970930pubmed:issnTypeElectroniclld:pubmed
pubmed-article:20970930pubmed:day24lld:pubmed
pubmed-article:20970930pubmed:volume148lld:pubmed
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pubmed-article:20970930pubmed:year2011lld:pubmed
pubmed-article:20970930pubmed:articleTitleIsolation and characterization of a reovirus causing spleen necrosis in Pekin ducklings.lld:pubmed
pubmed-article:20970930pubmed:affiliationKey Laboratory of Zoonosis of Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China.lld:pubmed
pubmed-article:20970930pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:20970930pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed