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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
2010-10-20
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pubmed:abstractText |
A group of SARS-like coronaviruses (SL-CoV) have been identified in horseshoe bats. Despite SL-CoVs and SARS-CoV share identical genome structure and high-level sequence similarity, SL-CoV does not bind to the same cellular receptor as for SARS-CoV and the N-terminus of the S proteins only share 64% amino acid identity, suggesting there are fundamental differences between these two groups of coronaviruses. To gain insight into the basis of this difference, we established a recombinant adenovirus system expressing the S protein from SL-CoV (rAd-Rp3-S) to investigate its immune characterization. Our results showed that immunized mice generated strong humoral immune responses against the SL-CoV S protein. Moreover, a strong cellular immune response demonstrated by elevated IFN-? and IL-6 levels was also observed in these mice. However, the induced antibody from these mice had weaker cross-reaction with the SARS-CoV S protein, and did not neutralize HIV pseudotyped with SARS-CoV S protein. These results demonstrated that the immunogenicity of the SL-CoV S protein is distinct from that of SARS-CoV, which may cause the immunological differences between human SARS-CoV and bat SL-CoV. Furthermore, the recombinant virus could serve as a potential vaccine candidate against bat SL-CoV infection.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Neutralizing,
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Viral,
http://linkedlifedata.com/resource/pubmed/chemical/Interferon-gamma,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-6,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Viral Envelope Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Viral Vaccines,
http://linkedlifedata.com/resource/pubmed/chemical/spike glycoprotein, coronavirus
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
1995-820X
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
25
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
36-44
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pubmed:meshHeading |
pubmed-meshheading:20960282-Adenoviridae,
pubmed-meshheading:20960282-Animals,
pubmed-meshheading:20960282-Antibodies, Neutralizing,
pubmed-meshheading:20960282-Antibodies, Viral,
pubmed-meshheading:20960282-Chiroptera,
pubmed-meshheading:20960282-Cross Reactions,
pubmed-meshheading:20960282-Female,
pubmed-meshheading:20960282-Gene Expression,
pubmed-meshheading:20960282-Genetic Vectors,
pubmed-meshheading:20960282-HIV,
pubmed-meshheading:20960282-Humans,
pubmed-meshheading:20960282-Interferon-gamma,
pubmed-meshheading:20960282-Interleukin-6,
pubmed-meshheading:20960282-Leukocytes, Mononuclear,
pubmed-meshheading:20960282-Membrane Glycoproteins,
pubmed-meshheading:20960282-Mice,
pubmed-meshheading:20960282-Mice, Inbred BALB C,
pubmed-meshheading:20960282-Neutralization Tests,
pubmed-meshheading:20960282-Recombinant Proteins,
pubmed-meshheading:20960282-SARS Virus,
pubmed-meshheading:20960282-Viral Envelope Proteins,
pubmed-meshheading:20960282-Viral Vaccines
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pubmed:year |
2010
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pubmed:articleTitle |
Immunogenicity of the spike glycoprotein of bat SARS-like coronavirus.
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pubmed:affiliation |
State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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