Source:http://linkedlifedata.com/resource/pubmed/id/20950592
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:dateCreated |
2010-12-20
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| pubmed:abstractText |
Various studies on focal cerebral ischemic models have implicated the direct activation and expression of matrix metalloproteinases (MMPs), especially MMP-9, as a key orchestrator of blood-brain barrier (BBB) disruption. Moreover, studies have shown that MMP-9 siRNA can protect the BBB from ischemia/reperfusion injury. In the present study, we investigated the neuroprotective role of a lentivirus vector-mediated mmp-9shRNA following focal cerebral ischemia--specifically assessing whether LV-mmp9shRNA silencing of MMP-9 mRNA could ameliorate BBB disruption and in turn reduce vascular permeability, neuronal cell death, and neurobehavioral deficits. Treatment was given 2 weeks prior to surgery using a lentivirus-mediated vector. Surgery was conducted using the established middle cerebral artery occlusion (MCAO) model in rats, while outcomes were measured 24 h after injury. Our results demonstrated a significant reduction in brain infarction volume, brain water content, and neurobehavioral deficits following LV-mmp9shRNA treatment. Additionally, Evans blue and IgG extravasation were reduced, MMP-9 mRNA expression was silenced, and Western blot analysis revealed a decreased expression of MMP-9 and VEGF with an increased expression of occludin and collagen IV in brain tissues. This suggests that successful delivery of LV-mmp9shRNA may ameliorate ischemic brain injury by preserving structural integrity and improving functional outcome.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Evans Blue,
http://linkedlifedata.com/resource/pubmed/chemical/Matrix Metalloproteinase 9,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Small Interfering,
http://linkedlifedata.com/resource/pubmed/chemical/Tetrazolium Salts,
http://linkedlifedata.com/resource/pubmed/chemical/triphenyltetrazolium
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| pubmed:status |
MEDLINE
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| pubmed:month |
Jan
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| pubmed:issn |
1872-6240
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| pubmed:author | |
| pubmed:copyrightInfo |
Copyright © 2010 Elsevier B.V. All rights reserved.
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| pubmed:issnType |
Electronic
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| pubmed:day |
7
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| pubmed:volume |
1367
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
347-59
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| pubmed:meshHeading |
pubmed-meshheading:20950592-Analysis of Variance,
pubmed-meshheading:20950592-Animals,
pubmed-meshheading:20950592-Blood-Brain Barrier,
pubmed-meshheading:20950592-Cell Line, Transformed,
pubmed-meshheading:20950592-Cells, Cultured,
pubmed-meshheading:20950592-Disease Models, Animal,
pubmed-meshheading:20950592-Evans Blue,
pubmed-meshheading:20950592-Gene Expression Regulation,
pubmed-meshheading:20950592-Gene Transfer Techniques,
pubmed-meshheading:20950592-Infarction, Middle Cerebral Artery,
pubmed-meshheading:20950592-Lentivirus,
pubmed-meshheading:20950592-Male,
pubmed-meshheading:20950592-Matrix Metalloproteinase 9,
pubmed-meshheading:20950592-RNA, Messenger,
pubmed-meshheading:20950592-RNA, Small Interfering,
pubmed-meshheading:20950592-Rats,
pubmed-meshheading:20950592-Rats, Sprague-Dawley,
pubmed-meshheading:20950592-Single-Blind Method,
pubmed-meshheading:20950592-Tetrazolium Salts,
pubmed-meshheading:20950592-Trauma Severity Indices
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| pubmed:year |
2011
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| pubmed:articleTitle |
Lentivirus-mediated transfer of MMP-9 shRNA provides neuroprotection following focal ischemic brain injury in rats.
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| pubmed:affiliation |
Department of Anatomy and Embryology, Peking University Health Science Center, Beijing 100191, China.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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