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PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
12
pubmed:dateCreated
2010-11-30
pubmed:databankReference
pubmed:abstractText
We analyzed a collection of 60 Salmonella enterica 4,5,12:i:- phage type U302 multidrug-resistant monophasic variant strains, isolated in Spain between 2000 and 2007. Most strains showed resistance to ampicillin (A), chloramphenicol (C), sulfamethoxazole (Su), gentamicin (G), streptomycin (S), tetracycline (T), and co-trimoxazole (SxT) (an ACSuGSTSxT resistance pattern). Only one pulsed-field gel electrophoresis (PFGE) type was detected, with 19 subtypes (Simpson's index of diversity [SID]=0.89). Multiple-locus variable-number tandem-repeat analysis (MLVA) showed more variability, with 32 profiles (SID=0.97), but only showed diversity at the STTR5 and STTR6 loci. PCR and sequencing demonstrated all strains contained the same allantoin-glyoxylate pathway deletion. Four types of deletions were detected in the fljAB operon, all starting at the same position, at the STM2758 gene, and followed by an IS26 insertion. Furthermore, a representative set of strains of the four deletion types harbored plasmids with IS26. We propose that a Salmonella enterica serotype Typhimurium U302 multidrug-resistant (ACSuGSTSxT) strain, defective for the allantoin-glyoxylate pathway and containing IS26 at plasmid pU302L, could be the ancestor of the variant in Spain.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/20943866-10488227, http://linkedlifedata.com/resource/pubmed/commentcorrection/20943866-11014799, http://linkedlifedata.com/resource/pubmed/commentcorrection/20943866-11034302, http://linkedlifedata.com/resource/pubmed/commentcorrection/20943866-11292803, http://linkedlifedata.com/resource/pubmed/commentcorrection/20943866-11474028, http://linkedlifedata.com/resource/pubmed/commentcorrection/20943866-12037067, http://linkedlifedata.com/resource/pubmed/commentcorrection/20943866-15071035, http://linkedlifedata.com/resource/pubmed/commentcorrection/20943866-15369852, http://linkedlifedata.com/resource/pubmed/commentcorrection/20943866-15746367, http://linkedlifedata.com/resource/pubmed/commentcorrection/20943866-16828159, http://linkedlifedata.com/resource/pubmed/commentcorrection/20943866-19741087, http://linkedlifedata.com/resource/pubmed/commentcorrection/20943866-3069867, http://linkedlifedata.com/resource/pubmed/commentcorrection/20943866-321679, http://linkedlifedata.com/resource/pubmed/commentcorrection/20943866-6312419, http://linkedlifedata.com/resource/pubmed/commentcorrection/20943866-7489730, http://linkedlifedata.com/resource/pubmed/commentcorrection/20943866-7793624
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
1098-660X
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
48
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
4563-6
pubmed:dateRevised
2011-7-28
pubmed:meshHeading
pubmed-meshheading:20943866-Allantoin, pubmed-meshheading:20943866-Anti-Bacterial Agents, pubmed-meshheading:20943866-Bacterial Typing Techniques, pubmed-meshheading:20943866-Bacteriophage Typing, pubmed-meshheading:20943866-Biosynthetic Pathways, pubmed-meshheading:20943866-DNA Transposable Elements, pubmed-meshheading:20943866-Drug Resistance, Multiple, Bacterial, pubmed-meshheading:20943866-Electrophoresis, Gel, Pulsed-Field, pubmed-meshheading:20943866-Evolution, Molecular, pubmed-meshheading:20943866-Gene Deletion, pubmed-meshheading:20943866-Glyoxylates, pubmed-meshheading:20943866-Humans, pubmed-meshheading:20943866-Minisatellite Repeats, pubmed-meshheading:20943866-Molecular Sequence Data, pubmed-meshheading:20943866-Molecular Typing, pubmed-meshheading:20943866-Mutagenesis, Insertional, pubmed-meshheading:20943866-Polymerase Chain Reaction, pubmed-meshheading:20943866-Salmonella Infections, pubmed-meshheading:20943866-Salmonella enterica, pubmed-meshheading:20943866-Salmonella typhimurium, pubmed-meshheading:20943866-Sequence Analysis, DNA, pubmed-meshheading:20943866-Serotyping, pubmed-meshheading:20943866-Spain
pubmed:year
2010
pubmed:articleTitle
Genetic evolution of the Spanish multidrug-resistant Salmonella enterica 4,5,12:i:- monophasic variant.
pubmed:affiliation
Department of Immunology, Microbiology, and Parasitology, Faculty of Pharmacy, University of the Basque Country, Paseo de la Universidad 7, 01006 Vitoria-Gasteiz, Spain.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't