|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
20
|
|
pubmed:dateCreated |
2010-11-9
|
|
pubmed:abstractText |
We aimed to clarify the transcription-regulation mechanisms of the mouse telomerase reverse transcriptase gene (mTERT). First, we searched for the promoter region required for transcriptional activation of mTERT and identified an enhancer cis-element (named mTERT-EE) located between -200 and -179bp of the mouse TERT gene (mTERT). EMSA results suggested that nuclear factor of activated T cells (NFAT) member proteins bind to mTERT-EE. We then identified NFAT5 as the factor binding to mTERT-EE and found that it activates the transcription of the mTERT core promoter. The results that siRNA directed against NFAT5 significantly reduced mTERT expression and mTERT core promoter activity and that the expressions of NFAT5 and mTERT were well correlated in various mouse tissues except liver suggest that NFAT5 dominantly and directly regulates mTERT expression. To clarify their functionality further, we investigated the effect of hypertonic stress, a known stimulus affecting the expression and transcriptional activity of NFAT5, on mTERT expression. The result indicated that hypertonic stress activates mTERT transcription via the activation and recruitment of NFAT5 to the mTERT promoter. These results provide useful information about the transcription-regulation mechanisms of mTERT.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Cell Extracts,
http://linkedlifedata.com/resource/pubmed/chemical/Hypertonic Solutions,
http://linkedlifedata.com/resource/pubmed/chemical/NFATC Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Nfat5 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Small Interfering,
http://linkedlifedata.com/resource/pubmed/chemical/Telomerase,
http://linkedlifedata.com/resource/pubmed/chemical/Tert protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Dec
|
|
pubmed:issn |
1090-2422
|
|
pubmed:author |
|
|
pubmed:copyrightInfo |
Copyright © 2010 Elsevier Inc. All rights reserved.
|
|
pubmed:issnType |
Electronic
|
|
pubmed:day |
10
|
|
pubmed:volume |
316
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
3342-50
|
|
pubmed:meshHeading |
pubmed-meshheading:20937271-Animal Structures,
pubmed-meshheading:20937271-Animals,
pubmed-meshheading:20937271-Binding Sites,
pubmed-meshheading:20937271-Cell Extracts,
pubmed-meshheading:20937271-Cell Nucleus,
pubmed-meshheading:20937271-Cell Survival,
pubmed-meshheading:20937271-Electrophoretic Mobility Shift Assay,
pubmed-meshheading:20937271-Enhancer Elements, Genetic,
pubmed-meshheading:20937271-Fibroblasts,
pubmed-meshheading:20937271-Gene Expression,
pubmed-meshheading:20937271-Gene Expression Regulation,
pubmed-meshheading:20937271-Genes, Reporter,
pubmed-meshheading:20937271-Hypertonic Solutions,
pubmed-meshheading:20937271-Mice,
pubmed-meshheading:20937271-Mice, Inbred Strains,
pubmed-meshheading:20937271-Mutation,
pubmed-meshheading:20937271-NFATC Transcription Factors,
pubmed-meshheading:20937271-NIH 3T3 Cells,
pubmed-meshheading:20937271-Promoter Regions, Genetic,
pubmed-meshheading:20937271-RNA, Small Interfering,
pubmed-meshheading:20937271-Stress, Physiological,
pubmed-meshheading:20937271-Telomerase,
pubmed-meshheading:20937271-Transcription, Genetic,
pubmed-meshheading:20937271-Transcription Factors
|
|
pubmed:year |
2010
|
|
pubmed:articleTitle |
NFAT5 regulates transcription of the mouse telomerase reverse transcriptase gene.
|
|
pubmed:affiliation |
Faculty of Agriculture, Kyushu University, Fukuoka, Japan.
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
