Linked Life Data

20923197

Source:http://linkedlifedata.com/resource/pubmed/id/20923197

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
12
pubmed:dateCreated
2010-12-3
pubmed:abstractText
Drought is one of the most severe limitations to plant growth and productivity. Resurrection plants have evolved a unique capability to tolerate desiccation in vegetative tissues. Fern-ally Selaginella tamariscina (Beauv.) is one of the most primitive vascular resurrection plants, which can survive a desiccated state and recover when water becomes available. To better understand the mechanism of desiccation tolerance, we have applied physiological and proteomic analysis. Samples of S. tamariscina were water-deprived for up to seven days followed by 12 h of rewatering. Our results showed that endogenous abscisic acid (ABA) increased to regulate dehydration-responsive genes/proteins and physiological processes. In the course of dehydration, the contents of osmolytes represented by soluble sugars and proline were increased to maintain cell structure integrity. The activities of four antioxidant enzymes (superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), and glutathione reductase (GR)) also increased. In contrast, both the rate of photosynthesis and the chlorophyll content decreased, and plasma membrane integrity was lost. We identified 138 desiccation-responsive two-dimensional electrophoresis (2-DE) spots, representing 103 unique proteins. Hierarchical clustering analysis revealed that 83% of the proteins were down-regulated upon dehydration. They were mainly involved in photosynthesis, carbohydrate and energy metabolism, stress and defense, protein metabolism, signaling, membrane/transport, cell structure, and cell division. The dynamic expression changes of the desiccation-responsive proteins provide strong evidence that cell structure modification, photosynthesis reduction, antioxidant system activation, and protein post-transcriptional/translational modifications are essential to the poikilochlorophyllous fern-ally S. tamariscina in response to dehydration. In addition, our comparative analysis of dehydration-responsive proteins in vegetative tissues from 19 desiccation tolerant and nontolerant plant species suggests that resurrection S. tamariscina has developed a specific desiccation tolerant mechanism. To our knowledge, this study constitutes the first detailed investigation of the protein complement in fern/fern-allies.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
1535-3907
pubmed:author
pubmed:issnType
Electronic
pubmed:day
3
pubmed:volume
9
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
6561-77
pubmed:meshHeading
pubmed-meshheading:20923197-Abscisic Acid, pubmed-meshheading:20923197-Adaptation, Physiological, pubmed-meshheading:20923197-Catalase, pubmed-meshheading:20923197-Chlorophyll, pubmed-meshheading:20923197-Electrophoresis, Gel, Two-Dimensional, pubmed-meshheading:20923197-Glutathione Reductase, pubmed-meshheading:20923197-Osmotic Pressure, pubmed-meshheading:20923197-Peroxidase, pubmed-meshheading:20923197-Photosynthesis, pubmed-meshheading:20923197-Plant Proteins, pubmed-meshheading:20923197-Proteome, pubmed-meshheading:20923197-Proteomics, pubmed-meshheading:20923197-Selaginellaceae, pubmed-meshheading:20923197-Spectrometry, Mass, Matrix-Assisted Laser..., pubmed-meshheading:20923197-Stress, Physiological, pubmed-meshheading:20923197-Superoxide Dismutase, pubmed-meshheading:20923197-Time Factors, pubmed-meshheading:20923197-Water
pubmed:year
2010
pubmed:articleTitle
Desiccation tolerance mechanism in resurrection fern-ally Selaginella tamariscina revealed by physiological and proteomic analysis.
pubmed:affiliation
Key Laboratory of Saline-alkali Vegetation Ecology Restoration in Oil Field, Ministry of Education, College of Life Sciences, Northeast Forestry University, Harbin 150040, China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't