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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
2010-10-5
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pubmed:abstractText |
Establishing synaptic connections often involves the activity-dependent withdrawal of off-target contacts. We describe an in vivo role for temporally patterned electrical activity, voltage-gated calcium channels, and CaMKII in modulating the response of Drosophila motoneurons to the chemorepellent Sema-2a during synaptic refinement. Mutations affecting the Sema-2a ligand, the plexin B receptor (plexB), the voltage-gated Ca(v)2.1 calcium channel (cac), or the voltage-gated Na(v)1 sodium channel (mle(nap-ts);tipE) each result in ectopic neuromuscular contacts. Sema-2a interacts genetically with both of the channel mutations. The cac phenotype is enhanced by the Sema-2a mutation and is suppressed by either plexB overexpression or patterned, low-frequency (0.01 Hz) bouts of electrical activity in the embryo. The calcium-dependent suppression of ectopic contacts also depends on the downstream activation of CaMKII. These results indicate a role for patterned electrical activity and presynaptic calcium signaling, acting through CaMKII, in modulating a retrograde signal during the refinement of synaptic connections.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium Channels, N-Type,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium-Calmodulin-Dependent...,
http://linkedlifedata.com/resource/pubmed/chemical/Cell Adhesion Molecules, Neuronal,
http://linkedlifedata.com/resource/pubmed/chemical/Drosophila Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Nerve Tissue Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Cell Surface,
http://linkedlifedata.com/resource/pubmed/chemical/Semaphorins,
http://linkedlifedata.com/resource/pubmed/chemical/fasciclin II,
http://linkedlifedata.com/resource/pubmed/chemical/plexB protein, Drosophila,
http://linkedlifedata.com/resource/pubmed/chemical/voltage-dependent calcium channel...
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
1097-4199
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pubmed:author |
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pubmed:copyrightInfo |
Copyright © 2010 Elsevier Inc. All rights reserved.
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pubmed:issnType |
Electronic
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pubmed:day |
6
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pubmed:volume |
68
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
32-44
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pubmed:dateRevised |
2011-10-6
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pubmed:meshHeading |
pubmed-meshheading:20920789-Action Potentials,
pubmed-meshheading:20920789-Animals,
pubmed-meshheading:20920789-Animals, Genetically Modified,
pubmed-meshheading:20920789-Calcium,
pubmed-meshheading:20920789-Calcium Channels, N-Type,
pubmed-meshheading:20920789-Calcium-Calmodulin-Dependent Protein Kinase Type 2,
pubmed-meshheading:20920789-Cell Adhesion Molecules, Neuronal,
pubmed-meshheading:20920789-Drosophila,
pubmed-meshheading:20920789-Drosophila Proteins,
pubmed-meshheading:20920789-Electric Stimulation,
pubmed-meshheading:20920789-Embryo, Nonmammalian,
pubmed-meshheading:20920789-Gene Expression Regulation, Developmental,
pubmed-meshheading:20920789-Genetic Testing,
pubmed-meshheading:20920789-Larva,
pubmed-meshheading:20920789-Motor Neurons,
pubmed-meshheading:20920789-Muscles,
pubmed-meshheading:20920789-Mutation,
pubmed-meshheading:20920789-Nerve Tissue Proteins,
pubmed-meshheading:20920789-Neuromuscular Junction,
pubmed-meshheading:20920789-Presynaptic Terminals,
pubmed-meshheading:20920789-Receptors, Cell Surface,
pubmed-meshheading:20920789-Semaphorins,
pubmed-meshheading:20920789-Signal Transduction
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pubmed:year |
2010
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pubmed:articleTitle |
Presynaptic activity and CaMKII modulate retrograde semaphorin signaling and synaptic refinement.
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pubmed:affiliation |
Pharmacology Department, Yale School of Medicine, New Haven, CT 06520, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
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