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rdf:type |
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lifeskim:mentions |
umls-concept:C0012632,
umls-concept:C0012854,
umls-concept:C0086418,
umls-concept:C1412215,
umls-concept:C1514562,
umls-concept:C1519249,
umls-concept:C1705165,
umls-concept:C1880389,
umls-concept:C1883204,
umls-concept:C1883221,
umls-concept:C2700061
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pubmed:issue |
20
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pubmed:dateCreated |
2010-10-18
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pubmed:abstractText |
The Z? domain of human ADAR1 (Z?(ADAR1)) preferentially binds Z-DNA rather than B-DNA with high binding affinity. Z?(ADAR1) binds to the Z-conformation of both non-CG-repeat DNA duplexes and a d(CGCGCG)(2) duplex similarly. We performed NMR experiments on complexes between the Z?(ADAR1) and non-CG-repeat DNA duplexes, d(CACGTG)(2) or d(CGTACG)(2), with a variety of protein-DNA molar ratios. Comparison of these results with those from the analysis of d(CGCGCG)(2) in the previous study suggests that Z?(ADAR1) exhibits the sequence preference of d(CGCGCG)(2)?d(CACGTG)(2)>d(CGTACG)(2) through multiple sequence discrimination steps during the B-Z transition.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
1873-3468
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pubmed:author |
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pubmed:copyrightInfo |
Copyright © 2010 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
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pubmed:issnType |
Electronic
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pubmed:day |
22
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pubmed:volume |
584
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
4344-50
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pubmed:meshHeading |
pubmed-meshheading:20875819-Adenosine Deaminase,
pubmed-meshheading:20875819-Algorithms,
pubmed-meshheading:20875819-Base Sequence,
pubmed-meshheading:20875819-Binding, Competitive,
pubmed-meshheading:20875819-Binding Sites,
pubmed-meshheading:20875819-Calorimetry,
pubmed-meshheading:20875819-DNA,
pubmed-meshheading:20875819-DNA, Z-Form,
pubmed-meshheading:20875819-Humans,
pubmed-meshheading:20875819-Kinetics,
pubmed-meshheading:20875819-Magnetic Resonance Spectroscopy,
pubmed-meshheading:20875819-Models, Chemical,
pubmed-meshheading:20875819-Nucleic Acid Conformation,
pubmed-meshheading:20875819-Oligonucleotides
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pubmed:year |
2010
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pubmed:articleTitle |
Sequence discrimination of the Z? domain of human ADAR1 during B-Z transition of DNA duplexes.
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pubmed:affiliation |
Department of Chemistry, RINS, Gyeongsang National University, Jinju, Gyengnam, Republic of Korea.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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