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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2010-11-29
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pubmed:abstractText |
Mutations in the PKHD1 gene result in autosomal recessive polycystic kidney disease (ARPKD) in humans. To determine the molecular mechanism of the cystogenesis in ARPKD, we recently generated a mouse model for ARPKD that carries a targeted mutation in the mouse orthologue of human PKHD1. The homozygous mutant mice display hepatorenal cysts whose phenotypes are similar to those of human ARPKD patients. By littermates of this mouse, we developed two immortalized renal collecting duct cell lines with Pkhd1 and two without. Under nonpermissive culture conditions, the Pkhd1(-/-) renal cells displayed aberrant cell-cell contacts and tubulomorphogenesis. The Pkhd1(-/-) cells also showed significantly reduced cell proliferation and elevated apoptosis. To validate this finding in vivo, we examined proliferation and apoptosis in the kidneys of Pkhd1(-/-) mice and their wildtype littermates. Using proliferation (PCNA and Histone-3) and apoptosis (TUNEL and caspase-3) markers, similar results were obtained in the Pkhd1(-/-) kidney tissues as in the cells. To identify the molecular basis of these findings, we analyzed the effect of Pkhd1 loss on multiple putative signaling regulators. We demonstrated that the loss of Pkhd1 disrupts multiple major phosphorylations of focal adhesion kinase (FAK), and these disruptions either inhibit the Ras/C-Raf pathways to suppress MEK/ERK activity and ultimately reduce cell proliferation, or suppress PDK1/AKT to upregulate Bax/caspase-9/caspase-3 and promote apoptosis. Our findings indicate that apoptosis may be a major player in the cyst formation in ARPKD, which may lead to new therapeutic strategies for human ARPKD.
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pubmed:grant |
http://linkedlifedata.com/resource/pubmed/grant/5P50 CA095103,
http://linkedlifedata.com/resource/pubmed/grant/DK062373,
http://linkedlifedata.com/resource/pubmed/grant/DK71090,
http://linkedlifedata.com/resource/pubmed/grant/R01 DK062373-01A1,
http://linkedlifedata.com/resource/pubmed/grant/R01 DK062373-02,
http://linkedlifedata.com/resource/pubmed/grant/R01 DK062373-03,
http://linkedlifedata.com/resource/pubmed/grant/R01 DK062373-04,
http://linkedlifedata.com/resource/pubmed/grant/R01 DK062373-05,
http://linkedlifedata.com/resource/pubmed/grant/R01 DK071090-01,
http://linkedlifedata.com/resource/pubmed/grant/R01 DK071090-02,
http://linkedlifedata.com/resource/pubmed/grant/R01 DK071090-03,
http://linkedlifedata.com/resource/pubmed/grant/R01 DK071090-04,
http://linkedlifedata.com/resource/pubmed/grant/R21 DK065111-01,
http://linkedlifedata.com/resource/pubmed/grant/R21 DK065111-02
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
1090-2422
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pubmed:author |
pubmed-author:CoffeyRobert JRJ,
pubmed-author:EckG WGW,
pubmed-author:HeXiushengX,
pubmed-author:HuBoB,
pubmed-author:LiCunxiC,
pubmed-author:LiangDanD,
pubmed-author:LobMM,
pubmed-author:QiuQingchaoQ,
pubmed-author:WuGuanqingG,
pubmed-author:ZhanQiminQ,
pubmed-author:ZhaoPingP
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pubmed:copyrightInfo |
Copyright © 2010 Elsevier Inc. All rights reserved.
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pubmed:issnType |
Electronic
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pubmed:day |
15
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pubmed:volume |
317
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
173-87
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pubmed:dateRevised |
2011-9-22
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pubmed:meshHeading |
pubmed-meshheading:20875407-Animals,
pubmed-meshheading:20875407-Apoptosis,
pubmed-meshheading:20875407-Caspase 3,
pubmed-meshheading:20875407-Caspase 9,
pubmed-meshheading:20875407-Cell Line, Transformed,
pubmed-meshheading:20875407-Cell Proliferation,
pubmed-meshheading:20875407-Crosses, Genetic,
pubmed-meshheading:20875407-Cysts,
pubmed-meshheading:20875407-Disease Models, Animal,
pubmed-meshheading:20875407-Epithelial Cells,
pubmed-meshheading:20875407-Genes, cdc,
pubmed-meshheading:20875407-Genotype,
pubmed-meshheading:20875407-Humans,
pubmed-meshheading:20875407-Kidney,
pubmed-meshheading:20875407-Kidney Tubules, Collecting,
pubmed-meshheading:20875407-Mice,
pubmed-meshheading:20875407-Mice, Congenic,
pubmed-meshheading:20875407-Mice, Inbred C57BL,
pubmed-meshheading:20875407-Mice, Knockout,
pubmed-meshheading:20875407-Mutation,
pubmed-meshheading:20875407-Phenotype,
pubmed-meshheading:20875407-Polycystic Kidney, Autosomal Recessive,
pubmed-meshheading:20875407-Proto-Oncogene Proteins c-akt,
pubmed-meshheading:20875407-Receptors, Cell Surface,
pubmed-meshheading:20875407-Signal Transduction
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pubmed:year |
2011
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pubmed:articleTitle |
Cystogenesis in ARPKD results from increased apoptosis in collecting duct epithelial cells of Pkhd1 mutant kidneys.
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pubmed:affiliation |
Cancer Research Institute, University of South China, Hengyang, Hunan, 421001, China.
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pubmed:publicationType |
Journal Article,
Comparative Study,
In Vitro,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
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