| pubmed-article:20855596 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:20855596 | lifeskim:mentions | umls-concept:C0027950 | lld:lifeskim |
| pubmed-article:20855596 | lifeskim:mentions | umls-concept:C0521449 | lld:lifeskim |
| pubmed-article:20855596 | lifeskim:mentions | umls-concept:C0029047 | lld:lifeskim |
| pubmed-article:20855596 | lifeskim:mentions | umls-concept:C1335439 | lld:lifeskim |
| pubmed-article:20855596 | lifeskim:mentions | umls-concept:C1704735 | lld:lifeskim |
| pubmed-article:20855596 | lifeskim:mentions | umls-concept:C2728259 | lld:lifeskim |
| pubmed-article:20855596 | pubmed:issue | 40 | lld:pubmed |
| pubmed-article:20855596 | pubmed:dateCreated | 2010-10-6 | lld:pubmed |
| pubmed-article:20855596 | pubmed:databankReference | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20855596 | pubmed:abstractText | Regulated polyadenylation is a broadly conserved mechanism that controls key events during oogenesis. Pivotal to that mechanism is GLD-2, a catalytic subunit of cytoplasmic poly(A) polymerase (PAP). Caenorhabditis elegans GLD-2 forms an active PAP with multiple RNA-binding partners to regulate diverse aspects of germline and early embryonic development. One GLD-2 partner, RNP-8, was previously shown to influence oocyte fate specification. Here we use a genomic approach to identify transcripts selectively associated with both GLD-2 and RNP-8. Among the 335 GLD-2/RNP-8 potential targets, most were annotated as germline mRNAs and many as maternal mRNAs. These targets include gld-2 and rnp-8 themselves, suggesting autoregulation. Removal of either GLD-2 or RNP-8 resulted in shortened poly(A) tails and lowered abundance of four target mRNAs (oma-2, egg-1, pup-2, and tra-2); GLD-2 depletion also lowered the abundance of most GLD-2/RNP-8 putative target mRNAs when assayed on microarrays. Therefore, GLD-2/RNP-8 appears to polyadenylate and stabilize its target mRNAs. We also provide evidence that rnp-8 influences oocyte development; rnp-8 null mutants have more germ cell corpses and fewer oocytes than normal. Furthermore, RNP-8 appears to work synergistically with another GLD-2-binding partner, GLD-3, to ensure normal oogenesis. We propose that the GLD-2/RNP-8 enzyme is a broad-spectrum regulator of the oogenesis program that acts within an RNA regulatory network to specify and produce fully functional oocytes. | lld:pubmed |
| pubmed-article:20855596 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20855596 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20855596 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20855596 | pubmed:language | eng | lld:pubmed |
| pubmed-article:20855596 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20855596 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:20855596 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20855596 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20855596 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20855596 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20855596 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20855596 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20855596 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20855596 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:20855596 | pubmed:month | Oct | lld:pubmed |
| pubmed-article:20855596 | pubmed:issn | 1091-6490 | lld:pubmed |
| pubmed-article:20855596 | pubmed:author | pubmed-author:KimbleJudithJ | lld:pubmed |
| pubmed-article:20855596 | pubmed:author | pubmed-author:KimKyung... | lld:pubmed |
| pubmed-article:20855596 | pubmed:author | pubmed-author:WilsonTracy... | lld:pubmed |
| pubmed-article:20855596 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:20855596 | pubmed:day | 5 | lld:pubmed |
| pubmed-article:20855596 | pubmed:volume | 107 | lld:pubmed |
| pubmed-article:20855596 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:20855596 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:20855596 | pubmed:pagination | 17445-50 | lld:pubmed |
| pubmed-article:20855596 | pubmed:meshHeading | pubmed-meshheading:20855596... | lld:pubmed |
| pubmed-article:20855596 | pubmed:meshHeading | pubmed-meshheading:20855596... | lld:pubmed |
| pubmed-article:20855596 | pubmed:meshHeading | pubmed-meshheading:20855596... | lld:pubmed |
| pubmed-article:20855596 | pubmed:meshHeading | pubmed-meshheading:20855596... | lld:pubmed |
| pubmed-article:20855596 | pubmed:meshHeading | pubmed-meshheading:20855596... | lld:pubmed |
| pubmed-article:20855596 | pubmed:meshHeading | pubmed-meshheading:20855596... | lld:pubmed |
| pubmed-article:20855596 | pubmed:meshHeading | pubmed-meshheading:20855596... | lld:pubmed |
| pubmed-article:20855596 | pubmed:meshHeading | pubmed-meshheading:20855596... | lld:pubmed |
| pubmed-article:20855596 | pubmed:meshHeading | pubmed-meshheading:20855596... | lld:pubmed |
| pubmed-article:20855596 | pubmed:meshHeading | pubmed-meshheading:20855596... | lld:pubmed |
| pubmed-article:20855596 | pubmed:meshHeading | pubmed-meshheading:20855596... | lld:pubmed |
| pubmed-article:20855596 | pubmed:meshHeading | pubmed-meshheading:20855596... | lld:pubmed |
| pubmed-article:20855596 | pubmed:year | 2010 | lld:pubmed |
| pubmed-article:20855596 | pubmed:articleTitle | GLD-2/RNP-8 cytoplasmic poly(A) polymerase is a broad-spectrum regulator of the oogenesis program. | lld:pubmed |
| pubmed-article:20855596 | pubmed:affiliation | Department of Biochemistry and Howard Hughes Medical Institute, University of Wisconsin, Madison, WI 53706, USA. | lld:pubmed |
| pubmed-article:20855596 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:20855596 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| pubmed-article:20855596 | pubmed:publicationType | Research Support, N.I.H., Extramural | lld:pubmed |
| entrez-gene:171627 | entrezgene:pubmed | pubmed-article:20855596 | lld:entrezgene |
| entrez-gene:172338 | entrezgene:pubmed | pubmed-article:20855596 | lld:entrezgene |
| http://linkedlifedata.com/r... | entrezgene:pubmed | pubmed-article:20855596 | lld:entrezgene |
| http://linkedlifedata.com/r... | entrezgene:pubmed | pubmed-article:20855596 | lld:entrezgene |
