Linked Life Data

20853344

Source:http://linkedlifedata.com/resource/pubmed/id/20853344

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2010-9-20
pubmed:abstractText
Adrenal medullary chromaffin granules (dense core secretory vesicles) have been a valuable model system for the study of the proteins and membrane components involved in the process of exocytosis. Because of the abundance of chromaffin granules in a readily available tissue source, bovine adrenal medullae, and their unique sedimentation properties, it is possible to obtain large quantities of highly purified granules and granule membranes in a short period of time. Two protocols are presented here for the isolation of chromaffin granules: a basic protocol based on differential centrifugation in an iso-osmotic medium that yields intact chromaffin granules, and an alternate protocol based on sedimentation through a density step gradient that provides a greater yield of more highly purified chromaffin granules. Since in the latter case the granules cannot be returned to a medium of physiological osmolarity without lysis after purification on the step gradient, the alternate protocol is more useful to obtain the granule membranes or contents for further study.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
1934-2616
pubmed:author
pubmed:copyrightInfo
© 2010 by John Wiley & Sons, Inc.
pubmed:issnType
Electronic
pubmed:volume
Chapter 3
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
Unit 3.39.1-10
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
Isolation of chromaffin granules.
pubmed:affiliation
Department of Pharmacology, University of Virginia, Charlottesville, Virginia, USA.
pubmed:publicationType
Journal Article