Linked Life Data

20838584

Source:http://linkedlifedata.com/resource/pubmed/id/20838584

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
9
pubmed:dateCreated
2010-9-14
pubmed:abstractText
The interaction between phytohormones is an important mechanism which controls growth and developmental processes in plants. Deciphering these interactions is a crucial step in helping to develop crops with enhanced yield and resistance to environmental stresses. Controlling the expression level of OsAP2-39 which includes an APETALA 2 (AP2) domain leads to phenotypic changes in rice. Overexpression of OsAP2-39 leads to a reduction in yield by decreasing the biomass and the number of seeds in the transgenic rice lines. Global transcriptome analysis of the OsAP2-39 overexpression transgenic rice revealed the upregulation of a key abscisic acid (ABA) biosynthetic gene OsNCED-I which codes for 9-cis-epoxycarotenoid dioxygenase and leads to an increase in the endogenous ABA level. In addition to OsNCED-1, the gene expression analysis revealed the upregulation of a gene that codes for the Elongation of Upper most Internode (EUI) protein, an enzyme that catalyzes 16?, 17-epoxidation of non-13-hydroxylated GAs, which has been shown to deactivate gibberellins (GAs) in rice. The exogenous application of GA restores the wild-type phenotype in the transgenic line and ABA application induces the expression of EUI and suppresses the expression of OsAP2-39 in the wild-type line. These observations clarify the antagonistic relationship between ABA and GA and illustrate a mechanism that leads to homeostasis of these hormones. In vivo and in vitro analysis showed that the expression of both OsNCED-1 and EUI are directly controlled by OsAP2-39. Together, these results reveal a novel mechanism for the control of the ABA/GA balance in rice which is regulated by OsAP2-39 that in turn regulates plant growth and seed production.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
1553-7404
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
6
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:dateRevised
2011-3-11
pubmed:meshHeading
pubmed-meshheading:20838584-Abscisic Acid, pubmed-meshheading:20838584-Adaptation, Physiological, pubmed-meshheading:20838584-Arabidopsis Proteins, pubmed-meshheading:20838584-Droughts, pubmed-meshheading:20838584-Flowers, pubmed-meshheading:20838584-Gene Expression Profiling, pubmed-meshheading:20838584-Gene Expression Regulation, Plant, pubmed-meshheading:20838584-Genes, Plant, pubmed-meshheading:20838584-Gibberellins, pubmed-meshheading:20838584-Homeodomain Proteins, pubmed-meshheading:20838584-Models, Biological, pubmed-meshheading:20838584-Nuclear Proteins, pubmed-meshheading:20838584-Oryza sativa, pubmed-meshheading:20838584-Phenotype, pubmed-meshheading:20838584-Phylogeny, pubmed-meshheading:20838584-Plant Leaves, pubmed-meshheading:20838584-Plant Proteins, pubmed-meshheading:20838584-Plant Roots, pubmed-meshheading:20838584-Plants, Genetically Modified, pubmed-meshheading:20838584-Protein Binding, pubmed-meshheading:20838584-Recombinant Proteins, pubmed-meshheading:20838584-Regulatory Sequences, Nucleic Acid, pubmed-meshheading:20838584-Sequence Analysis, DNA, pubmed-meshheading:20838584-Signal Transduction, pubmed-meshheading:20838584-Transcription Factors
pubmed:year
2010
pubmed:articleTitle
The APETALA-2-like transcription factor OsAP2-39 controls key interactions between abscisic acid and gibberellin in rice.
pubmed:affiliation
Department of Molecular and Cellular Biology, University of Guelph, Guelph, Ontario, Canada.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't