| pubmed-article:20826237 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:20826237 | lifeskim:mentions | umls-concept:C0033572 | lld:lifeskim |
| pubmed-article:20826237 | lifeskim:mentions | umls-concept:C0007595 | lld:lifeskim |
| pubmed-article:20826237 | lifeskim:mentions | umls-concept:C0033684 | lld:lifeskim |
| pubmed-article:20826237 | lifeskim:mentions | umls-concept:C0037083 | lld:lifeskim |
| pubmed-article:20826237 | lifeskim:mentions | umls-concept:C0077678 | lld:lifeskim |
| pubmed-article:20826237 | lifeskim:mentions | umls-concept:C1421293 | lld:lifeskim |
| pubmed-article:20826237 | lifeskim:mentions | umls-concept:C2700100 | lld:lifeskim |
| pubmed-article:20826237 | lifeskim:mentions | umls-concept:C1710082 | lld:lifeskim |
| pubmed-article:20826237 | lifeskim:mentions | umls-concept:C0851285 | lld:lifeskim |
| pubmed-article:20826237 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:20826237 | pubmed:dateCreated | 2011-1-31 | lld:pubmed |
| pubmed-article:20826237 | pubmed:abstractText | This study elucidates the role of E6-associated protein, E6-AP (a dual function steroid hormone receptor coactivator and ubiquitin-protein ligase) in the regulation of PI3K-Akt signaling pathway, prostate gland growth and proliferation. Here, we report the generation of transgenic mice and prostate cancer cell line, LNCaP cells that overexpress E6-AP protein. Using these models we show that the levels of total Akt and phosphorylated Akt (active Akt) are increased in E6-AP overexpressing prostate gland and LNCaP cells suggesting that E6-AP regulates the PI3K-Akt signaling pathway. The prostate glands in our transgenic mice are ~20% larger and produce preneoplastic lesions at the age of 18 months. Our data also suggest that E6-AP modulates PI3K-Akt signaling pathway by both androgen-independent and -dependent mechanisms. In the androgen-independent mechanism, E6-AP modulates PI3K-Akt signaling by regulating the protein levels of RhoA, a small GTPase, which is a negative regulator of the Akt signaling pathway. Further, we show that E6-AP, a known coactivator of AR, amplifies the androgen-dependent activation of PI3K-Akt signaling pathway. In addition, we show that stable overexpression of E6-AP in prostate cancer cells results in increased cell size and proliferation. Overall our data suggests that E6-AP regulates both the positive and negative modulators of the PI3K-Akt pathway in prostate cells which results in increased prostate cell growth, proliferation and decreased apoptosis.This article is part of a Special Issue entitled The 26S Proteasome: When degradation is just not enough! | lld:pubmed |
| pubmed-article:20826237 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:20826237 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20826237 | pubmed:language | eng | lld:pubmed |
| pubmed-article:20826237 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20826237 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:20826237 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20826237 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:20826237 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:20826237 | pubmed:month | Feb | lld:pubmed |
| pubmed-article:20826237 | pubmed:issn | 0006-3002 | lld:pubmed |
| pubmed-article:20826237 | pubmed:author | pubmed-author:NawazZafarZ | lld:pubmed |
| pubmed-article:20826237 | pubmed:author | pubmed-author:SrinivasanSat... | lld:pubmed |
| pubmed-article:20826237 | pubmed:copyrightInfo | Copyright © 2010 Elsevier B.V. All rights reserved. | lld:pubmed |
| pubmed-article:20826237 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:20826237 | pubmed:volume | 1809 | lld:pubmed |
| pubmed-article:20826237 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:20826237 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:20826237 | pubmed:pagination | 119-27 | lld:pubmed |
| pubmed-article:20826237 | pubmed:dateRevised | 2011-10-12 | lld:pubmed |
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| pubmed-article:20826237 | pubmed:meshHeading | pubmed-meshheading:20826237... | lld:pubmed |
| pubmed-article:20826237 | pubmed:year | 2011 | lld:pubmed |
| pubmed-article:20826237 | pubmed:articleTitle | E3 ubiquitin protein ligase, E6-associated protein (E6-AP) regulates PI3K-Akt signaling and prostate cell growth. | lld:pubmed |
| pubmed-article:20826237 | pubmed:affiliation | Department of Biochemistry & Molecular Biology, Universityof Miami Miller School of Medicine, Miami, FL 33136, USA. | lld:pubmed |
| pubmed-article:20826237 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:20826237 | pubmed:publicationType | Research Support, U.S. Gov't, Non-P.H.S. | lld:pubmed |
| pubmed-article:20826237 | pubmed:publicationType | Research Support, N.I.H., Extramural | lld:pubmed |
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