Linked Life Data

20822655

Source:http://linkedlifedata.com/resource/pubmed/id/20822655

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2010-9-8
pubmed:abstractText
A pressurized capillary electrochromatographic (pCEC) fingerprint of Ginkgo biloba leaf extract was developed on three different types of capillary columns. A commercial column packed with 3-microm particles and an in-house column packed with 5-microm particles were investigated for their performance. Additionally, a monolithic column was included in the fingerprint study as a potential alternative to the conventional packed columns. The effects of experimental parameters, such as the composition of the mobile phase, the concentration and pH of the buffer, and the applied voltage, were studied. Binary mobile phases consisting of acetonitrile and a 5 mM sodium dihydrogen phosphate electrolyte at pH 2.8 were used in gradient elution mode with an applied voltage of 5 kV. Under optimal gradient conditions, at least 45 peaks were observed within 60 min on the commercial packed column, whereas only about 20 peaks were separated on the methacrylate-based monolithic and the in-house packed columns. The commercial column thus clearly outperforms the two other. However, the properties of the monolithic stationary phase still might be adapted (i.e., by changing the polymerization-mixture composition, the porosity, and thus the selectivity of the phase might be changed), which could lead to an improved efficiency.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
1945-239X
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
48
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
428-35
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
Fingerprint development for Ginkgo biloba extracts by pressurized capillary electrochromatography: comparison of column types.
pubmed:affiliation
Department of Analytical Chemistry and Pharmaceutical Technology, Center for Pharmaceutical Research Pharmaceutical Institute, Vrije Universiteit Brussel-VUB, Laarbeeklaan 103, B-1090 Brussels, Belgium.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't