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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
11
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pubmed:dateCreated |
2010-10-26
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pubmed:abstractText |
The purpose of this study was to clarify the cytoprotective mechanism(s) induced in a conditionally immortalized syncytiotrophoblast cell line (TR-TBT 18d-1) exposed to hypertonic conditions. Hypertonicity-induced apoptosis of TR-TBT 18d-1 cells, but this was blocked by addition of 1 mM taurine to the culture medium. TauT-knockdown using siRNA revealed that TauT is a major contributor to taurine uptake by TR-TBT 18d-1 cells, at least under normal conditions. Cellular uptake of [(3)H]taurine and [(14)C]betaine by TR-TBT 18d-1 cells cultured under hypertonic conditions was increased compared to that under normal conditions. TauT, BGT-1, ATA2 and HSP70 mRNAs were upregulated by hypertonicity, while OCTN2, ENT1 and CNT1 mRNAs were downregulated. [(3)H]Taurine uptake was strongly inhibited by TauT inhibitors such as hypotaurine and ?-alanine. MeAIB, a system A specific substrate, inhibited hypertonic stress-induced [(14)C]betaine uptake. These results suggest that TauT and system A play cytoprotective roles in syncytiotrophoblasts exposed to hypertonic stress.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/2,2-dimethyl-beta-alanine,
http://linkedlifedata.com/resource/pubmed/chemical/Amino Acid Transport System A,
http://linkedlifedata.com/resource/pubmed/chemical/Betaine,
http://linkedlifedata.com/resource/pubmed/chemical/Hypertonic Solutions,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Transport Modulators,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Transport Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Small Interfering,
http://linkedlifedata.com/resource/pubmed/chemical/Taurine,
http://linkedlifedata.com/resource/pubmed/chemical/beta-Alanine,
http://linkedlifedata.com/resource/pubmed/chemical/taurine transporter
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
1532-3102
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pubmed:author |
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pubmed:copyrightInfo |
Copyright © 2010 Elsevier Ltd. All rights reserved.
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pubmed:issnType |
Electronic
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pubmed:volume |
31
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1003-9
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pubmed:meshHeading |
pubmed-meshheading:20801504-Amino Acid Transport System A,
pubmed-meshheading:20801504-Animals,
pubmed-meshheading:20801504-Apoptosis,
pubmed-meshheading:20801504-Betaine,
pubmed-meshheading:20801504-Biological Transport,
pubmed-meshheading:20801504-Cell Line,
pubmed-meshheading:20801504-Cytoprotection,
pubmed-meshheading:20801504-Gene Expression Regulation, Developmental,
pubmed-meshheading:20801504-Gene Silencing,
pubmed-meshheading:20801504-Hypertonic Solutions,
pubmed-meshheading:20801504-Membrane Glycoproteins,
pubmed-meshheading:20801504-Membrane Transport Modulators,
pubmed-meshheading:20801504-Membrane Transport Proteins,
pubmed-meshheading:20801504-RNA, Messenger,
pubmed-meshheading:20801504-RNA, Small Interfering,
pubmed-meshheading:20801504-Rats,
pubmed-meshheading:20801504-Stress, Physiological,
pubmed-meshheading:20801504-Taurine,
pubmed-meshheading:20801504-Trophoblasts,
pubmed-meshheading:20801504-beta-Alanine
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pubmed:year |
2010
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pubmed:articleTitle |
Roles of TauT and system A in cytoprotection of rat syncytiotrophoblast cell line exposed to hypertonic stress.
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pubmed:affiliation |
Faculty of Pharmacy, Keio University, 1-5-30 Shibakoen, Minato-ku, Tokyo 105-8512, Japan. nakashima-em@pha.keio.ac.jp
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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