Source:http://linkedlifedata.com/resource/pubmed/id/20799830
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
4
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pubmed:dateCreated |
2010-8-30
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pubmed:abstractText |
Dihydroartemisinin (DHA), a front-line antimalarial herbal compound, has been shown to possess promising anticancer activity with low toxicity. We have previously reported that DHA induced caspase-3-dependent apoptosis in human lung adenocarcinoma cells. However, the cellular target and molecular mechanism of DHA-induced apoptosis is still poorly defined. We use confocal fluorescence microscopy imaging, fluorescence resonance energy transfer, and fluorescence recovery after photobleaching techniques to explore the roles of DHA-elicited reactive oxygen species (ROS) in the DHA-induced Bcl-2 family proteins activation, mitochondrial dysfunction, caspase cascade, and cell death. Cell Counting Kit-8 assay and flow cytometry analysis showed that DHA induced ROS-mediated apoptosis. Confocal imaging analysis in a single living cell and Western blot assay showed that DHA triggered ROS-dependent Bax translocation, mitochondrial membrane depolarization, alteration of mitochondrial morphology, cytochrome c release, caspase-9, caspase-8, and caspase-3 activation, indicating the coexistence of ROS-mediated mitochondrial and death receptor pathway. Collectively, our findings demonstrate for the first time that DHA induces cell apoptosis by triggering ROS-mediated caspase-8/Bid activation and the mitochondrial pathway, which provides some novel insights into the application of DHA as a potential anticancer drug and a new therapeutic strategy by targeting ROS signaling in lung adenocarcinoma therapy in the future.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antimalarials,
http://linkedlifedata.com/resource/pubmed/chemical/Artemisinins,
http://linkedlifedata.com/resource/pubmed/chemical/Caspase 8,
http://linkedlifedata.com/resource/pubmed/chemical/Reactive Oxygen Species,
http://linkedlifedata.com/resource/pubmed/chemical/dihydroquinghaosu
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pubmed:status |
MEDLINE
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pubmed:issn |
1560-2281
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pubmed:author | |
pubmed:issnType |
Electronic
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pubmed:volume |
15
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
046028
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pubmed:meshHeading |
pubmed-meshheading:20799830-Adenocarcinoma,
pubmed-meshheading:20799830-Antimalarials,
pubmed-meshheading:20799830-Apoptosis,
pubmed-meshheading:20799830-Artemisinins,
pubmed-meshheading:20799830-Caspase 8,
pubmed-meshheading:20799830-Cell Line, Tumor,
pubmed-meshheading:20799830-Enzyme Activation,
pubmed-meshheading:20799830-Humans,
pubmed-meshheading:20799830-Microscopy, Fluorescence,
pubmed-meshheading:20799830-Mitochondria,
pubmed-meshheading:20799830-Reactive Oxygen Species,
pubmed-meshheading:20799830-Signal Transduction
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pubmed:articleTitle |
Single-cell analysis of dihydroartemisinin-induced apoptosis through reactive oxygen species-mediated caspase-8 activation and mitochondrial pathway in ASTC-a-1 cells using fluorescence imaging techniques.
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pubmed:affiliation |
South China Normal University, Institute of Laser Life Science, MOE Key Laboratory of Laser Life Science, Guangzhou, China.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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