Linked Life Data

20798585

Source:http://linkedlifedata.com/resource/pubmed/id/20798585

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
8
pubmed:dateCreated
2010-8-27
pubmed:abstractText
The recombinant DNA pLR5cat_PSAB in which pediocin PA-1 structural and immunity genes (pedAB) fused with the promoter and deduced signal sequence of an alpha-amylase gene from a bifidobacterial strain were inserted in pLR5cat, an Escherichia coli-lactobacilli shuttle vector was transferred to Lactobacillus reuteri KCTC 3679 and the transformant presented bacteriocin activity. The recombinant L. reuteri KCTC 3679 transformed with the shortened pLR5cat(S)_PSAB, where non-essential region for the lactobacilli replicon was removed, also showed bacteriocin activity. The molecular mass of the secreted pediocin PA-1 from the recombinant bacteria was the same as that of native pediocin PA-1 (~4.6 kDa) from Pediococcus acidilactici K10 on a sodium dodecylsulfate-polyacrylamide gel electrophoresis (SDS-PAGE) gel. In co-cultures with Listeria monocytogenes, the recombinant L. reuteri KCTC 3679 effectively reduced the viable cell count of the pathogenic bacterium by a 3 log scale compared with a control where L. monocytogenes was incubated alone.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
1017-7825
pubmed:author
pubmed:issnType
Print
pubmed:volume
20
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1215-8
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
Heterologous production of pediocin PA-1 in Lactobacillus reuteri.
pubmed:affiliation
Department of Biotechnology, Chungju National University, Jeungpyeong, Korea.
pubmed:publicationType
Journal Article