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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
10
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pubmed:dateCreated |
1991-5-1
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pubmed:abstractText |
Quantitative increases in HMG-CoA reductase, the rate-limiting enzyme in sterol biosynthesis, induce membrane biogenesis in both yeast and mammalian cells. The subcellular organization of the resulting membrane differs in the two cell types: mammalian cells generate crystalloid endoplasmic reticulum whereas yeast cells assemble karmellae. We examined the consequences of heterologous expression of HMG-CoA reductase to distinguish features of this response that were cell-type specific from those that were isozyme-specific. This analysis demonstrated that membrane proliferation was induced in both mammalian and yeast cells by HMG-CoA reductase from either organism. However, the morphology of the induced membranes was determined by the cell type rather than the particular isozyme. Thus, both yeast and mammalian HMG-CoA reductase contained functional signals for membrane proliferation that were operational in either cell type, but the qualitative response to those signals was cell-type specific.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
1043-4674
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
2
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pubmed:geneSymbol |
GAL1,
HMG1,
HMG2
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
915-21
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:2078559-Animals,
pubmed-meshheading:2078559-Cell Line,
pubmed-meshheading:2078559-Cercopithecus aethiops,
pubmed-meshheading:2078559-Cricetinae,
pubmed-meshheading:2078559-Endoplasmic Reticulum,
pubmed-meshheading:2078559-Fungal Proteins,
pubmed-meshheading:2078559-Genes, Fungal,
pubmed-meshheading:2078559-Genetic Complementation Test,
pubmed-meshheading:2078559-Humans,
pubmed-meshheading:2078559-Hydroxymethylglutaryl CoA Reductases,
pubmed-meshheading:2078559-Intracellular Membranes,
pubmed-meshheading:2078559-Isoenzymes,
pubmed-meshheading:2078559-Mammals,
pubmed-meshheading:2078559-Membrane Proteins,
pubmed-meshheading:2078559-Mesocricetus,
pubmed-meshheading:2078559-Organ Specificity,
pubmed-meshheading:2078559-Promoter Regions, Genetic,
pubmed-meshheading:2078559-Recombinant Fusion Proteins,
pubmed-meshheading:2078559-Saccharomyces cerevisiae
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pubmed:year |
1990
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pubmed:articleTitle |
Cell-type control of membrane biogenesis induced by HMG-CoA reductase.
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pubmed:affiliation |
University of California, Department of Molecular and Cellular Biology, Berkeley, CA 94720.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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