Source:http://linkedlifedata.com/resource/pubmed/id/20729841
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
9
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pubmed:dateCreated |
2010-8-31
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pubmed:abstractText |
We have developed a nematode transformation vector carrying the bacterial neomycin resistance gene (NeoR) and shown that it could confer resistance to G-418 on both wild-type Caenorhabditis elegans and C. briggsae. This selection system allows hands-off maintenance and enrichment of transgenic worms carrying non-integrated transgenes on selective plates. We also show that this marker can be used for Mos1-mediated single-copy insertion in wild-type genetic backgrounds (MosSCI-biotic).
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pubmed:commentsCorrections | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
1548-7105
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pubmed:author | |
pubmed:issnType |
Electronic
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pubmed:volume |
7
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
721-3
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pubmed:meshHeading |
pubmed-meshheading:20729841-Animals,
pubmed-meshheading:20729841-Animals, Genetically Modified,
pubmed-meshheading:20729841-Caenorhabditis,
pubmed-meshheading:20729841-Drug Resistance,
pubmed-meshheading:20729841-Genetic Markers,
pubmed-meshheading:20729841-Genetic Vectors,
pubmed-meshheading:20729841-Gentamicins,
pubmed-meshheading:20729841-Neomycin,
pubmed-meshheading:20729841-Selection, Genetic,
pubmed-meshheading:20729841-Transgenes
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pubmed:year |
2010
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pubmed:articleTitle |
An antibiotic selection marker for nematode transgenesis.
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pubmed:affiliation |
Genome Regulation and Evolution, INSERM U869, Institut Européen de Chimie et Biologie, Pessac, France.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
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