Linked Life Data

20719759

Source:http://linkedlifedata.com/resource/pubmed/id/20719759

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
11
pubmed:dateCreated
2010-10-13
pubmed:abstractText
Phospholipids are required for epidermal lamellar body formation. Glycerol 3-phosphate acyltransferases (GPATs) catalyze the initial step in the biosynthesis of glycerolipids. Little is known about the expression and regulation of GPATs in epidermis/keratinocytes. Here, we demonstrate that GPAT 1, 3, and 4 are expressed in epidermis/keratinocytes, whereas GPAT2 is not detected. In mouse epidermis, GPAT 3 and 4 are mainly localized to the upper layers whereas GPAT1 is found in both the upper and lower layers. GPAT1 and 3 mRNA increase during fetal rat epidermal development. No change in GPAT expression was observed in adult mice following acute permeability barrier disruption. Calcium-induced human keratinocyte differentiation increased GPAT3 mRNA whereas both GPAT1 and 4 mRNA levels decreased. In parallel, total GPAT activity increased 2-fold in differentiated keratinocytes attributable to an increase in N-ethylmaleimide (NEM) sensitive GPAT activity localized to microsomes with little change in NEM resistant activity, consistent with an increase in GPAT3. Furthermore, PPAR? or PPAR? activators increased GPAT3 mRNA, microsomal GPAT activity, and glycerol lipid synthesis without affecting the expression of GPAT1 or 4. Finally, both PPAR? and PPAR? activators increased GPAT3 mRNA via increasing its transcription. Thus, multiple isoforms of GPAT are expressed and differentially regulated in epidermis/keratinocytes.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0022-2275
pubmed:author
pubmed:issnType
Print
pubmed:volume
51
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3207-16
pubmed:dateRevised
2011-11-1
pubmed:meshHeading
pubmed-meshheading:20719759-Animals, pubmed-meshheading:20719759-Cell Differentiation, pubmed-meshheading:20719759-Cells, Cultured, pubmed-meshheading:20719759-Epidermis, pubmed-meshheading:20719759-Female, pubmed-meshheading:20719759-Gene Expression Regulation, Developmental, pubmed-meshheading:20719759-Gene Expression Regulation, Enzymologic, pubmed-meshheading:20719759-Glycerol-3-Phosphate O-Acyltransferase, pubmed-meshheading:20719759-Humans, pubmed-meshheading:20719759-Isoenzymes, pubmed-meshheading:20719759-Keratinocytes, pubmed-meshheading:20719759-Mice, pubmed-meshheading:20719759-Orphan Nuclear Receptors, pubmed-meshheading:20719759-Peroxisome Proliferator-Activated Receptors, pubmed-meshheading:20719759-Rats, pubmed-meshheading:20719759-Thiazolidinediones, pubmed-meshheading:20719759-Transcriptional Activation
pubmed:year
2010
pubmed:articleTitle
Expression and regulation of GPAT isoforms in cultured human keratinocytes and rodent epidermis.
pubmed:affiliation
Department of R&D, System Biosciences, Mountain View, CA 94043, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, N.I.H., Extramural