|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
6
|
|
pubmed:dateCreated |
2010-9-3
|
|
pubmed:abstractText |
The role APCs play in the transition of T cells from effector to memory remains largely undefined. This is likely due to the low frequency at which long-lived T cells arise, which hinders analysis of the events involved in memory development. In this study, we used TCR transgenic T cells to increase the frequency of long-lived T cells and developed a transfer model suitable for defining the contribution of APCs to the development of CD4 T cell memory. Accordingly, naive TCR transgenic T cells were stimulated in vitro with Ag presented by different types of APCs and transferred into MHC class II-deficient mice for parking, and the hosts were later analyzed for long-lived T cell frequency or challenged with suboptimal dose of Ag, and the long-lived cells-driven memory responses were measured. The findings indicate that B cells and CD8alpha(+) dendritic cells sustained elevated frequencies of long-lived T cells that yielded rapid and robust memory responses upon rechallenge with suboptimal dose of Ag. Furthermore, both types of APCs had significant programmed death (PD) ligand 2 expression prior to Ag stimulation, which was maintained at a high level during presentation of Ag to T cells. Blockade of PD ligand 2 interaction with its receptor PD-1 nullified the development of memory responses. These previously unrecognized findings suggest that targeting specific APCs for Ag presentation during vaccination could prove effective against microbial infections.
|
|
pubmed:grant |
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
AIM
|
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD,
http://linkedlifedata.com/resource/pubmed/chemical/Apoptosis Regulatory Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Histocompatibility Antigens Class II,
http://linkedlifedata.com/resource/pubmed/chemical/Intercellular Signaling Peptides...,
http://linkedlifedata.com/resource/pubmed/chemical/OVA 323-339,
http://linkedlifedata.com/resource/pubmed/chemical/Ovalbumin,
http://linkedlifedata.com/resource/pubmed/chemical/PDCD1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/PDCD1LG2 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Pdcd1lg2 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Peptide Fragments,
http://linkedlifedata.com/resource/pubmed/chemical/Programmed Cell Death 1 Ligand 2...,
http://linkedlifedata.com/resource/pubmed/chemical/Programmed Cell Death 1 Receptor
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Sep
|
|
pubmed:issn |
1550-6606
|
|
pubmed:author |
pubmed-author:CascioJason AJA,
pubmed-author:DhakalMermagyaM,
pubmed-author:EllisJason SJS,
pubmed-author:GulogluF BetulFB,
pubmed-author:HaymakerCara LCL,
pubmed-author:HoemanChristine MCM,
pubmed-author:TartarDanielle MDM,
pubmed-author:VanMorlanAmieA,
pubmed-author:XiaoxiaoWanW,
pubmed-author:YahngSeung-HiSH,
pubmed-author:ZaghouaniHabibH
|
|
pubmed:issnType |
Electronic
|
|
pubmed:day |
15
|
|
pubmed:volume |
185
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
3149-57
|
|
pubmed:dateRevised |
2011-11-17
|
|
pubmed:meshHeading |
pubmed-meshheading:20709947-Amino Acid Sequence,
pubmed-meshheading:20709947-Animals,
pubmed-meshheading:20709947-Antigen-Presenting Cells,
pubmed-meshheading:20709947-Antigens, CD,
pubmed-meshheading:20709947-Apoptosis Regulatory Proteins,
pubmed-meshheading:20709947-CD4-Positive T-Lymphocytes,
pubmed-meshheading:20709947-Cell Communication,
pubmed-meshheading:20709947-Cell Differentiation,
pubmed-meshheading:20709947-Chickens,
pubmed-meshheading:20709947-G0 Phase,
pubmed-meshheading:20709947-Histocompatibility Antigens Class II,
pubmed-meshheading:20709947-Immunologic Memory,
pubmed-meshheading:20709947-Intercellular Signaling Peptides and Proteins,
pubmed-meshheading:20709947-Mice,
pubmed-meshheading:20709947-Mice, Inbred BALB C,
pubmed-meshheading:20709947-Mice, Inbred C57BL,
pubmed-meshheading:20709947-Mice, Transgenic,
pubmed-meshheading:20709947-Molecular Sequence Data,
pubmed-meshheading:20709947-Ovalbumin,
pubmed-meshheading:20709947-Peptide Fragments,
pubmed-meshheading:20709947-Programmed Cell Death 1 Ligand 2 Protein,
pubmed-meshheading:20709947-Programmed Cell Death 1 Receptor
|
|
pubmed:year |
2010
|
|
pubmed:articleTitle |
APCs expressing high levels of programmed death ligand 2 sustain the development of CD4 T cell memory.
|
|
pubmed:affiliation |
Department of Molecular Microbiology and Immunology, University of Missouri School of Medicine, Columbia, MO 65212, USA.
|
|
pubmed:publicationType |
Journal Article,
Research Support, N.I.H., Extramural
|
