Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2010-8-31
pubmed:abstractText
The malignant Hodgkin/Reed-Sternberg (HRS) cells of Hodgkin lymphoma (HL) are believed to derive from germinal center (GC) B cells, but lack expression of a functional B cell receptor. As apoptosis is the normal fate of B-cell receptor-negative GC B cells, mechanisms that abrogate apoptosis are thus critical in HL development, such as epigenetic disruption of certain pro-apoptotic cancer genes including tumor suppressor genes. Identifying methylated genes elucidates oncogenic mechanisms and provides valuable biomarkers; therefore, we performed a chemical epigenetic screening for methylated genes in HL through pharmacological demethylation and expression profiling. IGSF4/CADM1/TSLC1, a pro-apoptotic cell adhesion molecule of the immunoglobulin superfamily, was identified together with other methylated targets. In contrast to its expression in normal GC B cells, IGSF4 was down-regulated and methylated in HL cell lines, most primary HL, and microdissected HRS cells of 3/5 cases, but not in normal peripheral blood mononuclear cells and seldom in normal lymph nodes. We also detected IGSF4 methylation in sera of 14/18 (78%) HL patients but seldom in normal sera. Ectopic IGSF4 expression decreased HL cells survival and increased their sensitivity to apoptosis. IGSF4 induction that normally follows heat shock stress treatment was also abrogated in methylated lymphoma cells. Thus, our data demonstrate that IGSF4 silencing by CpG methylation provides an anti-apoptotic signal to HRS cells important in HL pathogenesis.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/20709797-10344738, http://linkedlifedata.com/resource/pubmed/commentcorrection/20709797-10519413, http://linkedlifedata.com/resource/pubmed/commentcorrection/20709797-10570189, http://linkedlifedata.com/resource/pubmed/commentcorrection/20709797-10616196, http://linkedlifedata.com/resource/pubmed/commentcorrection/20709797-11036080, http://linkedlifedata.com/resource/pubmed/commentcorrection/20709797-11062252, http://linkedlifedata.com/resource/pubmed/commentcorrection/20709797-11175354, http://linkedlifedata.com/resource/pubmed/commentcorrection/20709797-11244505, http://linkedlifedata.com/resource/pubmed/commentcorrection/20709797-11279526, http://linkedlifedata.com/resource/pubmed/commentcorrection/20709797-11309499, http://linkedlifedata.com/resource/pubmed/commentcorrection/20709797-11571644, http://linkedlifedata.com/resource/pubmed/commentcorrection/20709797-11992124, http://linkedlifedata.com/resource/pubmed/commentcorrection/20709797-12042769, http://linkedlifedata.com/resource/pubmed/commentcorrection/20709797-12050160, http://linkedlifedata.com/resource/pubmed/commentcorrection/20709797-12154410, http://linkedlifedata.com/resource/pubmed/commentcorrection/20709797-12189161, http://linkedlifedata.com/resource/pubmed/commentcorrection/20709797-12213729, http://linkedlifedata.com/resource/pubmed/commentcorrection/20709797-12234973, http://linkedlifedata.com/resource/pubmed/commentcorrection/20709797-12498717, http://linkedlifedata.com/resource/pubmed/commentcorrection/20709797-12499253, http://linkedlifedata.com/resource/pubmed/commentcorrection/20709797-12582260, http://linkedlifedata.com/resource/pubmed/commentcorrection/20709797-12710672, http://linkedlifedata.com/resource/pubmed/commentcorrection/20709797-12756225, http://linkedlifedata.com/resource/pubmed/commentcorrection/20709797-12853486, http://linkedlifedata.com/resource/pubmed/commentcorrection/20709797-12942568, http://linkedlifedata.com/resource/pubmed/commentcorrection/20709797-12973698, http://linkedlifedata.com/resource/pubmed/commentcorrection/20709797-1373383, http://linkedlifedata.com/resource/pubmed/commentcorrection/20709797-14500387, http://linkedlifedata.com/resource/pubmed/commentcorrection/20709797-14528273, http://linkedlifedata.com/resource/pubmed/commentcorrection/20709797-14559817, http://linkedlifedata.com/resource/pubmed/commentcorrection/20709797-14645011, http://linkedlifedata.com/resource/pubmed/commentcorrection/20709797-14672555, http://linkedlifedata.com/resource/pubmed/commentcorrection/20709797-14961078, http://linkedlifedata.com/resource/pubmed/commentcorrection/20709797-14970278, http://linkedlifedata.com/resource/pubmed/commentcorrection/20709797-15057748, http://linkedlifedata.com/resource/pubmed/commentcorrection/20709797-15077158, http://linkedlifedata.com/resource/pubmed/commentcorrection/20709797-15184878, http://linkedlifedata.com/resource/pubmed/commentcorrection/20709797-15592515, http://linkedlifedata.com/resource/pubmed/commentcorrection/20709797-15705871, http://linkedlifedata.com/resource/pubmed/commentcorrection/20709797-15905536, http://linkedlifedata.com/resource/pubmed/commentcorrection/20709797-16166418, http://linkedlifedata.com/resource/pubmed/commentcorrection/20709797-16223734, http://linkedlifedata.com/resource/pubmed/commentcorrection/20709797-16229803, http://linkedlifedata.com/resource/pubmed/commentcorrection/20709797-16247458, http://linkedlifedata.com/resource/pubmed/commentcorrection/20709797-16261159, http://linkedlifedata.com/resource/pubmed/commentcorrection/20709797-16439445, http://linkedlifedata.com/resource/pubmed/commentcorrection/20709797-16491070, http://linkedlifedata.com/resource/pubmed/commentcorrection/20709797-17967063, http://linkedlifedata.com/resource/pubmed/commentcorrection/20709797-18469857, http://linkedlifedata.com/resource/pubmed/commentcorrection/20709797-18794147, http://linkedlifedata.com/resource/pubmed/commentcorrection/20709797-19147580, http://linkedlifedata.com/resource/pubmed/commentcorrection/20709797-8879220, http://linkedlifedata.com/resource/pubmed/commentcorrection/20709797-8977533, http://linkedlifedata.com/resource/pubmed/commentcorrection/20709797-9786912
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
1525-2191
pubmed:author
pubmed:issnType
Electronic
More...