| pubmed-article:20700128 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:20700128 | lifeskim:mentions | umls-concept:C0225336 | lld:lifeskim |
| pubmed-article:20700128 | lifeskim:mentions | umls-concept:C0302600 | lld:lifeskim |
| pubmed-article:20700128 | lifeskim:mentions | umls-concept:C0228174 | lld:lifeskim |
| pubmed-article:20700128 | lifeskim:mentions | umls-concept:C1456820 | lld:lifeskim |
| pubmed-article:20700128 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:20700128 | pubmed:dateCreated | 2011-2-4 | lld:pubmed |
| pubmed-article:20700128 | pubmed:abstractText | Erythropoietin (EPO) enhances angiogenesis in the ischemic brain. Stroke induces secretion of tumor necrosis factor ? (TNF-?). We investigated the effect of TNF-? on EPO-induced in vitro angiogenesis in cerebral endothelial cells. Using a capillary-like tubular formation assay, we found that transient incubation of primary rat cerebral microvascular endothelial cells (RECs) with TNF-? substantially upregulated EPO receptor (EPOR) expression and addition of EPO into TNF-?-treated RECs significantly augmented the capillary-like tube formation. Blockage of TNF receptor 1 (TNFR1) suppressed TNF-?-upregulated EPOR expression and abolished EPO-induced tube formation. Attenuation of endogenous EPOR with small interfering RNA (siRNA) also inhibited EPO-enhanced tube formation. Treatment of RECs with EPO activated nuclear factor-kappa B (NF-?B) and Akt. Incubation of the TNF-?-treated endothelial cells with EPO activated vascular endothelial growth factor (VEGF), VEGF receptor 2 (VEGFR2), angiopoietin 1 (Ang1), and Tie2. Blockage of VEGFR2 and Tie2 resulted in reduction of EPO-augmented tube formation. These data indicate that interaction of TNF-? with TNFR1 sensitizes cerebral endothelial cells for EPO-induced angiogenesis by upregulation of EPOR, which amplifies the effect of EPO on activation of the VEGF/VEGFR2 and Ang1/Tie2 pathways. Our results provide the evidence for crosslink between TNF and EPOR to coordinate the onset of angiogenesis in cerebral endothelial cells. | lld:pubmed |
| pubmed-article:20700128 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20700128 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20700128 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20700128 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20700128 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20700128 | pubmed:language | eng | lld:pubmed |
| pubmed-article:20700128 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20700128 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:20700128 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20700128 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20700128 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20700128 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20700128 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20700128 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20700128 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20700128 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20700128 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20700128 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:20700128 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:20700128 | pubmed:month | Feb | lld:pubmed |
| pubmed-article:20700128 | pubmed:issn | 1559-7016 | lld:pubmed |
| pubmed-article:20700128 | pubmed:author | pubmed-author:SwanL CLC | lld:pubmed |
| pubmed-article:20700128 | pubmed:author | pubmed-author:WangLeiL | lld:pubmed |
| pubmed-article:20700128 | pubmed:author | pubmed-author:ChoppMichaelM | lld:pubmed |
| pubmed-article:20700128 | pubmed:author | pubmed-author:ZhangZheng... | lld:pubmed |
| pubmed-article:20700128 | pubmed:author | pubmed-author:ZhangRui... | lld:pubmed |
| pubmed-article:20700128 | pubmed:author | pubmed-author:SagerThomas... | lld:pubmed |
| pubmed-article:20700128 | pubmed:author | pubmed-author:SzaladAlexand... | lld:pubmed |
| pubmed-article:20700128 | pubmed:author | pubmed-author:Moniche-Alvar... | lld:pubmed |
| pubmed-article:20700128 | pubmed:author | pubmed-author:BolzMarianneM | lld:pubmed |
| pubmed-article:20700128 | pubmed:author | pubmed-author:FranciscoMoni... | lld:pubmed |
| pubmed-article:20700128 | pubmed:author | pubmed-author:AluigiDaniell... | lld:pubmed |
| pubmed-article:20700128 | pubmed:author | pubmed-author:WangXin LiXL | lld:pubmed |
| pubmed-article:20700128 | pubmed:author | pubmed-author:ChrsitensenSø... | lld:pubmed |
| pubmed-article:20700128 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:20700128 | pubmed:volume | 31 | lld:pubmed |
| pubmed-article:20700128 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:20700128 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:20700128 | pubmed:pagination | 640-7 | lld:pubmed |
| pubmed-article:20700128 | pubmed:dateRevised | 2011-8-1 | lld:pubmed |
| pubmed-article:20700128 | pubmed:meshHeading | pubmed-meshheading:20700128... | lld:pubmed |
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| pubmed-article:20700128 | pubmed:year | 2011 | lld:pubmed |
| pubmed-article:20700128 | pubmed:articleTitle | Tumor necrosis factor ? primes cerebral endothelial cells for erythropoietin-induced angiogenesis. | lld:pubmed |
| pubmed-article:20700128 | pubmed:affiliation | Department of Neurology, Henry Ford Health Sciences Center, Detroit, Michigan 48202, USA. | lld:pubmed |
| pubmed-article:20700128 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:20700128 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| pubmed-article:20700128 | pubmed:publicationType | Research Support, N.I.H., Extramural | lld:pubmed |
| entrez-gene:25625 | entrezgene:pubmed | pubmed-article:20700128 | lld:entrezgene |
