Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
35
pubmed:dateCreated
2010-9-2
pubmed:abstractText
We report observations suggesting that the transcription elongation factor NusA promotes a previously unrecognized class of transcription-coupled repair (TCR) in addition to its previously proposed role in recruiting translesion synthesis (TLS) DNA polymerases to gaps encountered during transcription. Earlier, we reported that NusA physically and genetically interacts with the TLS DNA polymerase DinB (DNA pol IV). We find that Escherichia coli nusA11(ts) mutant strains, at the permissive temperature, are highly sensitive to nitrofurazone (NFZ) and 4-nitroquinolone-1-oxide but not to UV radiation. Gene expression profiling suggests that this sensitivity is unlikely to be due to an indirect effect on gene expression affecting a known DNA repair or damage tolerance pathway. We demonstrate that an N(2)-furfuryl-dG (N(2)-f-dG) lesion, a structural analog of the principal lesion generated by NFZ, blocks transcription by E. coli RNA polymerase (RNAP) when present in the transcribed strand, but not when present in the nontranscribed strand. Our genetic analysis suggests that NusA participates in a nucleotide excision repair (NER)-dependent process to promote NFZ resistance. We provide evidence that transcription plays a role in the repair of NFZ-induced lesions through the isolation of RNAP mutants that display altered ability to survive NFZ exposure. We propose that NusA participates in an alternative class of TCR involved in the identification and removal of a class of lesion, such as the N(2)-f-dG lesion, which are accurately and efficiently bypassed by DinB in addition to recruiting DinB for TLS at gaps encountered by RNAP.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/20696893-11040219, http://linkedlifedata.com/resource/pubmed/commentcorrection/20696893-11683261, http://linkedlifedata.com/resource/pubmed/commentcorrection/20696893-12086674, http://linkedlifedata.com/resource/pubmed/commentcorrection/20696893-12756229, http://linkedlifedata.com/resource/pubmed/commentcorrection/20696893-1339317, http://linkedlifedata.com/resource/pubmed/commentcorrection/20696893-1370474, http://linkedlifedata.com/resource/pubmed/commentcorrection/20696893-15249207, http://linkedlifedata.com/resource/pubmed/commentcorrection/20696893-15387814, http://linkedlifedata.com/resource/pubmed/commentcorrection/20696893-15690043, http://linkedlifedata.com/resource/pubmed/commentcorrection/20696893-16091045, http://linkedlifedata.com/resource/pubmed/commentcorrection/20696893-16407906, http://linkedlifedata.com/resource/pubmed/commentcorrection/20696893-16452972, http://linkedlifedata.com/resource/pubmed/commentcorrection/20696893-16452982, http://linkedlifedata.com/resource/pubmed/commentcorrection/20696893-16645050, http://linkedlifedata.com/resource/pubmed/commentcorrection/20696893-17581590, http://linkedlifedata.com/resource/pubmed/commentcorrection/20696893-1763073, http://linkedlifedata.com/resource/pubmed/commentcorrection/20696893-17889665, http://linkedlifedata.com/resource/pubmed/commentcorrection/20696893-18487194, http://linkedlifedata.com/resource/pubmed/commentcorrection/20696893-18562283, http://linkedlifedata.com/resource/pubmed/commentcorrection/20696893-18697749, http://linkedlifedata.com/resource/pubmed/commentcorrection/20696893-18788757, http://linkedlifedata.com/resource/pubmed/commentcorrection/20696893-18996995, http://linkedlifedata.com/resource/pubmed/commentcorrection/20696893-19150431, http://linkedlifedata.com/resource/pubmed/commentcorrection/20696893-19465649, http://linkedlifedata.com/resource/pubmed/commentcorrection/20696893-19680289, http://linkedlifedata.com/resource/pubmed/commentcorrection/20696893-19895820, http://linkedlifedata.com/resource/pubmed/commentcorrection/20696893-19948952, http://linkedlifedata.com/resource/pubmed/commentcorrection/20696893-20016485, http://linkedlifedata.com/resource/pubmed/commentcorrection/20696893-20016488, http://linkedlifedata.com/resource/pubmed/commentcorrection/20696893-20036541, http://linkedlifedata.com/resource/pubmed/commentcorrection/20696893-20505728, http://linkedlifedata.com/resource/pubmed/commentcorrection/20696893-20733069, http://linkedlifedata.com/resource/pubmed/commentcorrection/20696893-2250027, http://linkedlifedata.com/resource/pubmed/commentcorrection/20696893-2443722, http://linkedlifedata.com/resource/pubmed/commentcorrection/20696893-2554145, http://linkedlifedata.com/resource/pubmed/commentcorrection/20696893-2821282, http://linkedlifedata.com/resource/pubmed/commentcorrection/20696893-3664636, http://linkedlifedata.com/resource/pubmed/commentcorrection/20696893-3838150, http://linkedlifedata.com/resource/pubmed/commentcorrection/20696893-4612337, http://linkedlifedata.com/resource/pubmed/commentcorrection/20696893-4865486, http://linkedlifedata.com/resource/pubmed/commentcorrection/20696893-4926026, http://linkedlifedata.com/resource/pubmed/commentcorrection/20696893-6090455, http://linkedlifedata.com/resource/pubmed/commentcorrection/20696893-6263495, http://linkedlifedata.com/resource/pubmed/commentcorrection/20696893-641373, http://linkedlifedata.com/resource/pubmed/commentcorrection/20696893-6758952, http://linkedlifedata.com/resource/pubmed/commentcorrection/20696893-8078911, http://linkedlifedata.com/resource/pubmed/commentcorrection/20696893-8341674, http://linkedlifedata.com/resource/pubmed/commentcorrection/20696893-8377190, http://linkedlifedata.com/resource/pubmed/commentcorrection/20696893-8465200, http://linkedlifedata.com/resource/pubmed/commentcorrection/20696893-8598198, http://linkedlifedata.com/resource/pubmed/commentcorrection/20696893-8757401, http://linkedlifedata.com/resource/pubmed/commentcorrection/20696893-8942666, http://linkedlifedata.com/resource/pubmed/commentcorrection/20696893-9016630
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/4-Nitroquinoline-1-oxide, http://linkedlifedata.com/resource/pubmed/chemical/4-nitroquinolone-1-oxide, http://linkedlifedata.com/resource/pubmed/chemical/Anti-Infective Agents, http://linkedlifedata.com/resource/pubmed/chemical/DNA-Directed RNA Polymerases, http://linkedlifedata.com/resource/pubmed/chemical/Escherichia coli Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Green Fluorescent Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Nitrofurazone, http://linkedlifedata.com/resource/pubmed/chemical/Peptide Elongation Factors, http://linkedlifedata.com/resource/pubmed/chemical/Quinolones, http://linkedlifedata.com/resource/pubmed/chemical/RNA polymerase alpha subunit, http://linkedlifedata.com/resource/pubmed/chemical/Rec A Recombinases, http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors, http://linkedlifedata.com/resource/pubmed/chemical/nusA protein, E coli
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
1091-6490
pubmed:author
pubmed:issnType
Electronic
pubmed:day
31
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