Linked Life Data

20694833

Source:http://linkedlifedata.com/resource/pubmed/id/20694833

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2011-2-2
pubmed:abstractText
Restriction fragment length analysis of PCR amplified 16S rDNA with AluI revealed the presence of a 265 bp fragment in all species of Bacillus with the exception of B. cereus and B. thuringiensis, which contains two restriction sites within this fragment which results in three smaller fragments totalling to 265 bp. Some distant species of Bacillus with no evidence of this fragment could be delineated into other genera based on phenotypic and genotypic parameters. BLAST search for homologous sequences of individual species revealed that it is a highly conserved region. Multiple alignment of the fragment suggests that a region between 160 and 265 bp of the 265 bp fragment was a hypervariable region and were highly species-specific. A set of primers was designed for amplification of this hypervariable region. Partial sequencing of the hypervariable region within the 265 bp fragment seems an index for identification of Bacillus species.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
1572-9699
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
99
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
283-96
pubmed:meshHeading
pubmed:year
2011
pubmed:articleTitle
Restriction analysis and partial sequencing of the 16S rRNA gene as index for rapid identification of Bacillus species.
pubmed:affiliation
National Bureau of Agriculturally Important Microorganisms, Kusmaur, Mau Nath Bhanjan, UP, India. teeny_vardhan@yahoo.co.in
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't, Evaluation Studies