Linked Life Data

20691864

Source:http://linkedlifedata.com/resource/pubmed/id/20691864

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2010-8-9
pubmed:abstractText
Experimental mouse chimeras have served as immensely important research tools for studying many aspects of mammalian development ever since they first were produced over 50 years ago. Chimera studies have served as crucial assays in the era of modern mouse genetics that was triggered by the advent of mouse embryonic stem cells. Lately, chimeras are also used as proof of pluripotency and normality of induced pluripotent stem cells. With this long history in mind, it may seem surprising that chimeras now have an ever-increasing role to play. The high-throughput mouse gene targeting projects are in the process of producing ES cell lines with a mutation in each of the close to 20,000 known protein coding genes. These will all be waiting for germline transmission through chimeras. Such a large-scale approach calls for simplified methods for generating germline transmitting chimeras. In this chapter, we will describe the currently most cost efficient and simple method; the aggregation of pluripotent stem cells with diploid or tetraploid mouse embryos. Since most of the large knockout projects are using the C57BL/6 background, we will pay special attention to cell lines derived from this inbred strain.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:issn
1557-7988
pubmed:author
pubmed:copyrightInfo
Copyright (c) 2010 Elsevier Inc. All rights reserved.
pubmed:issnType
Electronic
pubmed:volume
476
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
123-49
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
Production of mouse chimeras by aggregating pluripotent stem cells with embryos.
pubmed:affiliation
Mount Sinai Hospital, Samuel Lunenfeld Research Institute,Toronto, Ontario, Canada.
pubmed:publicationType
Journal Article