Linked Life Data

20682335

Source:http://linkedlifedata.com/resource/pubmed/id/20682335

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2010-9-14
pubmed:abstractText
Type B 8-keto trichothecenes are muco-active mycotoxins that are notable contaminants in cereal-based food stuffs. Epithelial responses are of primary concern during gastrointestinal exposure in human and animal intoxications. Therefore, optimized biomarkers to assess the specific action of trichothecenes on the human epithelial barrier are needed. In the present study, 8-keto trichothecenes were tested to trigger endoplasmic reticulum (ER) stresses that are evident as alteration of eukaryotic initiation factors 2? phosphorylation and other ER stress markers in human intestinal epithelial cells. Based on the ER stress-inducing activity of 8-keto trichothecenes, we developed a bio-monitoring method using intestinal epithelial cells constitutively expressing secretory alkaline phosphatase (SEAP) reporter, which can be used as a sensitive and selective indicator for monitoring ER stress in the epithelial barrier. Type B 8-keto trichothecenes suppressed the release of SEAP in a dose-dependent manner, showing a negative correlation between logarithm of toxin dose and SEAP activity. However, tested toxins did not affect SEAP enzymatic activity and mRNA levels, but reduced the cellular release of SEAP. Moreover, in order to correct the monitoring of 8-keto trichothecene in crop matrix, the bioassay was proven to work in standard cereal extract of corn and rice. The mechanism-based monitoring of 8-keto trichothecenes is promising as a supplementary analysis means of the presence of bioactive 8-keto trichothecenes, which are potentially exposed in human gut epithelia.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
1879-3169
pubmed:author
pubmed:copyrightInfo
Copyright 2010 Elsevier Ireland Ltd. All rights reserved.
pubmed:issnType
Electronic
pubmed:day
20
pubmed:volume
198
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
317-23
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
Mechanism-based alternative monitoring of endoplasmic reticulum stress by 8-keto-trichothecene mycotoxins using human intestinal epithelial cell line.
pubmed:affiliation
Department of Microbiology and Immunology, and Medical Research Institute, Pusan National University School of Medicine, Yangsan 626-813, Republic of Korea.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't