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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
2010-8-30
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pubmed:abstractText |
Phospholipase C-gamma1 (PLC-gamma1), a tyrosine kinase substrate, has been implicated in the pathway for the epidermal growth factor receptor (EGFR)-induced cell migration. However, the underlying mechanism by which PLC-gamma1 mediates EGFR-induced cell migration remains elusive. In the present study, we sought to determine whether the lipase activity of PLC-gamma1 is required for EGFR-induced cell migration. We found that overexpression of PLC-gamma1 in squamous cell carcinoma SCC4 cells markedly enhanced EGF-induced PLC-gamma1 activation, intracellular calcium rise, and cell migration. This enhancement was abolished by mutational inactivation of the catalytic domain of PLC-gamma1. Inhibition of the downstream signaling processes mediated by the activity of phospholipase C (PLC) using IP(3) receptor inhibitor or intracellular calcium chelator blocked EGF-induced cell migration. These data indicate that EGF-induced cell migration is mediated by the lipase domain of PLC-gamma1 and the subsequent IP(3) generation and intracellular calcium mobilization.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/20674545-10069594,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20674545-10400667,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20674545-10400829,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20674545-10404636,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20674545-10554021,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20674545-10579907,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20674545-10975856,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20674545-11710940,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20674545-12354693,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20674545-14500405,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20674545-15604992,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20674545-15872086,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20674545-1683701,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20674545-17242406,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20674545-1748821,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20674545-18594017,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20674545-19158393,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20674545-19632319,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20674545-20215102,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20674545-20510673,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20674545-20590612,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20674545-2147038,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20674545-2338654,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20674545-3838314,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20674545-7962064,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20674545-8275435,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20674545-9265648,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20674545-9496939,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20674545-9686621
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
1090-2104
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pubmed:author |
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pubmed:copyrightInfo |
Copyright 2010 Elsevier Inc. All rights reserved.
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pubmed:issnType |
Electronic
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pubmed:day |
27
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pubmed:volume |
399
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
425-8
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pubmed:dateRevised |
2011-8-30
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pubmed:meshHeading |
pubmed-meshheading:20674545-Calcium,
pubmed-meshheading:20674545-Catalysis,
pubmed-meshheading:20674545-Cell Line, Tumor,
pubmed-meshheading:20674545-Cell Movement,
pubmed-meshheading:20674545-Epidermal Growth Factor,
pubmed-meshheading:20674545-Humans,
pubmed-meshheading:20674545-Inositol 1,4,5-Trisphosphate Receptors,
pubmed-meshheading:20674545-Neoplasm Invasiveness,
pubmed-meshheading:20674545-Phospholipase C gamma,
pubmed-meshheading:20674545-Receptor, Epidermal Growth Factor
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pubmed:year |
2010
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pubmed:articleTitle |
Critical role for the catalytic activity of phospholipase C-gamma1 in epidermal growth factor-induced cell migration.
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pubmed:affiliation |
Endocrine Unit, Veterans Affairs Medical Center, Northern California Institute for Research and Education, University of California, San Francisco, CA 94121, USA. Zhongjian.Xie@gmail.com
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pubmed:publicationType |
Journal Article,
Research Support, N.I.H., Extramural
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