Linked Life Data

20658137

Source:http://linkedlifedata.com/resource/pubmed/id/20658137

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2011-1-17
pubmed:abstractText
In order to examine the mediatory role of proton motive force (?p) or proton ATPase in H? production by Rhodobacter sphaeroides, ?p was determined under anaerobic conditions in the dark, and the ATPase activity has been studied in R. sphaeroides strain A-10, isolated from Arzni mineral springs in Armenia. Membrane potential (??) was measured from the distribution of tetraphenylphosphonium cation; pH gradient (?pH) was the difference between the external and cytoplasmic pH values, and the latter was measured by 9-aminoacridine (9-AA) fluorescence changes. At pH 7.5, ?? was of -94 mV and the reversed ?pH was +30 mV, resulting in ?p of -64 mV. The addition of N,N'-dicyclohexylcarbodiimide (DCCD), the F?F?-ATPase inhibitor, was not affect ??. It was shown that ?? varies nearly linearly with ?pH, ?? increased from -57.1 mV at pH 6.0 to -103.8 mV at pH 8.0; it was compensated at high external pH by a reversed ?pH, resulting in a low ?p under anaerobic-dark conditions. Intracellular ATP concentrations and energetic charge (EC) were measured to evaluate a metabolism activity of R. sphaeroides.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
1432-0991
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
62
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
415-9
pubmed:meshHeading
pubmed:year
2011
pubmed:articleTitle
Proton motive force in Rhodobacter sphaeroides under anaerobic conditions in the dark.
pubmed:affiliation
Department of Biophysics, Biological Faculty, Yerevan State University, 1 Alex Manoukian Str, 0025, Yerevan, Armenia.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't