Source:http://linkedlifedata.com/resource/pubmed/id/20647560
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
4
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pubmed:dateCreated |
2010-10-4
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pubmed:abstractText |
Engagement of 2B4 (CD244) with CD48 results in activation, costimulation, or inhibition of NK cell activities, depending on the cell types and the stage of differentiation. In vivo, 2B4+ NK cells can interact with CD48+ NK cells and also with surrounding CD48+ hematopoietic cells. Similarly, CD48+ NK cells may be triggered by adjacent 2B4+ NK cells or other hematopoietic cells expressing 2B4, e.g., monocytes, basophils, ?? T cells, etc. As CD48 was also shown to function as an activating receptor, 2B4/CD48 binding in the settings of NK-to-NK or NK-to-non-NK cell interactions may generate bidirectional signals. To address this question, we examined the consequence of CD48 or 2B4 ligation using two experimental settings: one with target (syngeneic EL4 and allogeneic P815) cells, ectopically expressing surface 2B4 or CD48, and the other with direct cross-linking with plate-bound mAb. Here, we report that ligation of CD48 with 2B4+ EL4 or 2B4+ P815 targets, in the absence of other receptor engagement, did not alter NK cell cytotoxicity or proliferation significantly. Similarly, cross-linking of NK cells with plate-bound anti-CD48 mAb in the absence or presence of a suboptimal dose of IL-2 did not modulate NK proliferation, cytotoxicity, or cytokine production. Nonetheless, 2B4 cross-linking promoted NK cell proliferation and effector functions consistently in both settings. Therefore, our results demonstrate unequivocally that CD48 on surrounding NK or non-NK cells serves primarily as a ligand to stimulate 2B4 on the adjacent NK cells in mice.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
1938-3673
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pubmed:author | |
pubmed:issnType |
Electronic
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pubmed:volume |
88
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
707-14
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pubmed:meshHeading |
pubmed-meshheading:20647560-Animals,
pubmed-meshheading:20647560-Antigens, CD,
pubmed-meshheading:20647560-Cell Separation,
pubmed-meshheading:20647560-Cytotoxicity, Immunologic,
pubmed-meshheading:20647560-Female,
pubmed-meshheading:20647560-Flow Cytometry,
pubmed-meshheading:20647560-Killer Cells, Natural,
pubmed-meshheading:20647560-Lymphocyte Activation,
pubmed-meshheading:20647560-Mice,
pubmed-meshheading:20647560-Mice, Inbred C57BL,
pubmed-meshheading:20647560-Receptors, Immunologic,
pubmed-meshheading:20647560-Signal Transduction
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pubmed:year |
2010
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pubmed:articleTitle |
Unidirectional signaling triggered through 2B4 (CD244), not CD48, in murine NK cells.
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pubmed:affiliation |
Global Research Lab, Department of Biochemistry and Division of Brain Korea 21 Program for Biomedical Science, Graduate School of Medicine, Korea University College of Medicine, Seoul, Korea.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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