Source:http://linkedlifedata.com/resource/pubmed/id/20639866
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
8
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| pubmed:dateCreated |
2010-8-10
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| pubmed:abstractText |
A fundamental goal in biotechnology and biology is the development of approaches to better understand the genetic basis of traits. Here we report a versatile method, trackable multiplex recombineering (TRMR), whereby thousands of specific genetic modifications are created and evaluated simultaneously. To demonstrate TRMR, in a single day we modified the expression of >95% of the genes in Escherichia coli by inserting synthetic DNA cassettes and molecular barcodes upstream of each gene. Barcode sequences and microarrays were then used to quantify population dynamics. Within a week we mapped thousands of genes that affect E. coli growth in various media (rich, minimal and cellulosic hydrolysate) and in the presence of several growth inhibitors (beta-glucoside, D-fucose, valine and methylglyoxal). This approach can be applied to a broad range of traits to identify targets for future genome-engineering endeavors.
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| pubmed:commentsCorrections | |
| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Aug
|
| pubmed:issn |
1546-1696
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| pubmed:author | |
| pubmed:issnType |
Electronic
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| pubmed:volume |
28
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
856-62
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| pubmed:meshHeading |
pubmed-meshheading:20639866-Base Sequence,
pubmed-meshheading:20639866-Computational Biology,
pubmed-meshheading:20639866-DNA Barcoding, Taxonomic,
pubmed-meshheading:20639866-Escherichia coli,
pubmed-meshheading:20639866-Escherichia coli Proteins,
pubmed-meshheading:20639866-Gene Expression Profiling,
pubmed-meshheading:20639866-Gene Expression Regulation, Bacterial,
pubmed-meshheading:20639866-Gene Library,
pubmed-meshheading:20639866-Genetic Engineering,
pubmed-meshheading:20639866-Genome, Bacterial,
pubmed-meshheading:20639866-Molecular Sequence Data,
pubmed-meshheading:20639866-Mutation,
pubmed-meshheading:20639866-Oligonucleotide Array Sequence Analysis,
pubmed-meshheading:20639866-Oligonucleotides,
pubmed-meshheading:20639866-Recombination, Genetic,
pubmed-meshheading:20639866-Sequence Analysis, DNA
|
| pubmed:year |
2010
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| pubmed:articleTitle |
Rapid profiling of a microbial genome using mixtures of barcoded oligonucleotides.
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| pubmed:affiliation |
Department of Chemical and Biological Engineering, University of Colorado, Boulder, Colorado, USA.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|