Linked Life Data

20637246

Source:http://linkedlifedata.com/resource/pubmed/id/20637246

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2010-8-23
pubmed:abstractText
In this study we demonstrate RNA interference mediated knock-down of target gene expression in Echinococcus multilocularis primary cells on both the transcriptional and translational level. In addition, we report on an improved method for generating E. multilocularis primary cell mini-aggregates from in vitro cultivated metacestode vesicles, and on the cultivation of small numbers of small interfering RNA-transfected cells in vitro over an extended period of time. This allows assessments on the effects of RNA interference performed on Echinococcus primary cells with regard to growth, proliferation, differentiation of the parasite and the formation of novel metacestode vesicles in vitro.
pubmed:commentsCorrections
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
1872-9428
pubmed:author
pubmed:copyrightInfo
Copyright 2010 Elsevier B.V. All rights reserved.
pubmed:issnType
Electronic
pubmed:volume
174
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
83-7
pubmed:dateRevised
2011-3-30
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
Echinococcus multilocularis primary cells: improved isolation, small-scale cultivation and RNA interference.
pubmed:affiliation
Institute of Parasitology, University of Berne, Länggass-Strasse 122, CH-3012 Berne, Switzerland. markus.spiliotis@ipa.unibe.ch
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't