|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:dateCreated |
2010-7-15
|
|
pubmed:abstractText |
One limit on developing complex synthetic gene circuits is the lack of basic components such as transcriptional logic gates that can process combinatorial inputs. Here, we propose a strategy to construct such components based on reusable designs and convergent reengineering of well-studied natural systems. We demonstrated the strategy using variants of the transcription factor (TF) LacI and operator Olac that form specifically interacting pairs. Guided by a mathematical model derived from existing quantitative knowledge, rational designs of transcriptional NAND, NOR and NOT gates have been realized. The NAND gates have been designed based on direct protein-protein interactions in coupling with DNA looping. We demonstrated that the designs are reusable: a multiplex of logic devices can be readily created using the same designs but different combinations of sequence variants. The designed logic gates are combinable to form compound circuits: a demonstration logic circuit containing all three types of designed logic gates has been synthesized, and the circuit truthfully reproduces the pre-designed input-output logic relations.
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/20631682-10659856,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20631682-10669616,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20631682-12065400,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20631682-12432407,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20631682-12702751,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20631682-12787501,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20631682-15797194,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20631682-15950160,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20631682-16094693,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20631682-16738662,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20631682-16785444,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20631682-17700541,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20631682-17928170,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20631682-18004278,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20631682-18075675,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20631682-18355019,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20631682-18414488,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20631682-18657511,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20631682-18971928,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20631682-19060164,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20631682-19148099,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20631682-19377462,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20631682-2182324,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20631682-2523839,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20631682-2663473,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20631682-6316325,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20631682-7792597,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20631682-8632456,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20631682-8638105,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20631682-9430644
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Jul
|
|
pubmed:issn |
1744-4292
|
|
pubmed:author |
|
|
pubmed:issnType |
Electronic
|
|
pubmed:day |
13
|
|
pubmed:volume |
6
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
388
|
|
pubmed:meshHeading |
pubmed-meshheading:20631682-Amino Acid Sequence,
pubmed-meshheading:20631682-Base Sequence,
pubmed-meshheading:20631682-Computer-Aided Design,
pubmed-meshheading:20631682-DNA,
pubmed-meshheading:20631682-Electronics,
pubmed-meshheading:20631682-Escherichia coli,
pubmed-meshheading:20631682-Gene Regulatory Networks,
pubmed-meshheading:20631682-Lac Operon,
pubmed-meshheading:20631682-Models, Genetic,
pubmed-meshheading:20631682-Molecular Sequence Data,
pubmed-meshheading:20631682-Operator Regions, Genetic,
pubmed-meshheading:20631682-Proteins,
pubmed-meshheading:20631682-Systems Biology,
pubmed-meshheading:20631682-Transcription Factors
|
|
pubmed:year |
2010
|
|
pubmed:articleTitle |
Develop reusable and combinable designs for transcriptional logic gates.
|
|
pubmed:affiliation |
School of Life Sciences, University of Science and Technology of China, Hefei, Anhui, China.
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
