|
rdf:type |
|
|
lifeskim:mentions |
umls-concept:C0013117,
umls-concept:C0030012,
umls-concept:C0037083,
umls-concept:C0040649,
umls-concept:C0441655,
umls-concept:C0596235,
umls-concept:C0851285,
umls-concept:C0851827,
umls-concept:C1336789,
umls-concept:C1701901,
umls-concept:C1710082,
umls-concept:C1825644
|
|
pubmed:issue |
7
|
|
pubmed:dateCreated |
2011-3-9
|
|
pubmed:abstractText |
DREAM/KChIP3 (Downstream Regulatory Element Antagonist Modulator) is a multifunctional Ca(2+)-binding protein that acts in the nucleus as a Ca(2+)-dependent transcriptional repressor. Binding to DNA and repressor activity of DREAM is regulated by Ca(2+), specific post-translational modifications as well as by protein-protein interactions with several nucleoproteins. Here, using the yeast two-hybrid assay, we characterized the interaction of DREAM with peroxiredoxin 3 (Prdx3), an antioxidant enzyme that uses the thioredoxin system as electron donor. Importantly, the DREAM/Prdx3 interaction is Ca(2+) dependent and is blocked by DTT. Coexpression of Prdx3 enhances DREAM binding to DRE sites and its repressor activity in vivo. Two cysteine residues in the N-terminal domain of DREAM are responsible for the redox modulation of its activity. Double Cys to Ser substitution results in a mutant DREAM with stronger repressor activity. Finally, we show that transient DREAM knockdown sensitizes PC12 cells to H(2)O(2)-induced oxidative stress, suggesting a protective role for DREAM against oxidative damage.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Apr
|
|
pubmed:issn |
1557-7716
|
|
pubmed:author |
|
|
pubmed:issnType |
Electronic
|
|
pubmed:day |
1
|
|
pubmed:volume |
14
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
1237-43
|
|
pubmed:meshHeading |
pubmed-meshheading:20618065-Animals,
pubmed-meshheading:20618065-Apoptosis,
pubmed-meshheading:20618065-CHO Cells,
pubmed-meshheading:20618065-COS Cells,
pubmed-meshheading:20618065-Cell Survival,
pubmed-meshheading:20618065-Cercopithecus aethiops,
pubmed-meshheading:20618065-Cricetinae,
pubmed-meshheading:20618065-Cricetulus,
pubmed-meshheading:20618065-Cysteine,
pubmed-meshheading:20618065-Gene Expression Regulation,
pubmed-meshheading:20618065-Gene Knockdown Techniques,
pubmed-meshheading:20618065-Kv Channel-Interacting Proteins,
pubmed-meshheading:20618065-Mutation,
pubmed-meshheading:20618065-Neurons,
pubmed-meshheading:20618065-Oxidation-Reduction,
pubmed-meshheading:20618065-Oxidative Stress,
pubmed-meshheading:20618065-PC12 Cells,
pubmed-meshheading:20618065-Peroxiredoxins,
pubmed-meshheading:20618065-Protein Binding,
pubmed-meshheading:20618065-Rats,
pubmed-meshheading:20618065-Reactive Oxygen Species,
pubmed-meshheading:20618065-Recombinant Proteins,
pubmed-meshheading:20618065-Regulatory Elements, Transcriptional,
pubmed-meshheading:20618065-Signal Transduction,
pubmed-meshheading:20618065-Two-Hybrid System Techniques
|
|
pubmed:year |
2011
|
|
pubmed:articleTitle |
Redox signaling regulates transcriptional activity of the Ca2+-dependent repressor DREAM.
|
|
pubmed:affiliation |
Dpto. Biología Molecular y Celular, Centro Nacional de Biotecnología, C.S.I.C., Madrid, Spain.
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
