Linked Life Data

20617828

Source:http://linkedlifedata.com/resource/pubmed/id/20617828

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
15
pubmed:dateCreated
2010-8-4
pubmed:abstractText
The antiviral properties of pressurized liquid extracts (PLE) (acetone, ethanol, and water) obtained from the edible microalga Chlorella vulgaris were evaluated against herpes simplex virus type 1 (HSV-1). None of the extracts tested showed extracellular direct virucidal activity against the virus, although a pretreatment of Vero cells with 75 microg/mL of water and ethanol extracts before virus addition inhibited 70% of the virus infection. Moreover, water and ethanol extracts were able to significantly inhibit the in vitro virus replication, showing IC(50%) values of 61.05 and 80.23 microg/mL respectively. To identify the type of compounds responsible for the antiviral activity found in the water extract, the polysaccharide fraction was isolated. This activity was found to correlate with polysaccharides, because the polysaccharide-rich fraction (46% concentrated) showed higher antiviral activity than the complete water extract. A concentration of 75 microg/mL of this fraction inhibited 90% virus infection when added as a pretreatment and showed an IC(50%) value of 33.93 microg/mL for intracellular virus replication. GC-MS characterization of the ethanol extract showed that the antiviral activity of this extract could be partially related with the presence of phytol, although other compounds could be involved in this activity.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
1520-5118
pubmed:author
pubmed:issnType
Electronic
pubmed:day
11
pubmed:volume
58
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
8522-7
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
Pressurized liquid extraction as an alternative process to obtain antiviral agents from the edible microalga Chlorella vulgaris.
pubmed:affiliation
Sección Departamental Ciencias de la Alimentación, Universidad Autónoma de Madrid, 28049 Madrid, Spain. susana.santoyo@uam.es
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't